Document Detail


Expression and roles of Slit/Robo in human ovarian cancer.
MedLine Citation:
PMID:  21465248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Slit glycoproteins and their Roundabout (Robo) receptors regulate migration and growth of many types of cells including human cancer cells. However, little is known about the expression and roles of Slit/Robo in human ovarian cancer. Herein, we examined the expression of Slit/Robo in human normal and malignant ovarian tissues and its potential participation in regulating migration and proliferation of human ovarian cancer cells using two ovarian cancer cell lines, OVCAR-3 and SKOV-3. We demonstrated that Slit2/3 and Robo1 were immunolocalized primarily in stromal cells in human normal ovaries and in cancer cells in many histotypes of ovarian cancer tissues. Protein expression of Slit2/3 and Robo1/4 was also identified in OVCAR-3 and SKOV-3 cells. However, recombinant human Slit2 did not significantly affect SKOV-3 cell migration, and OVCAR-3 and SKOV-3 cell proliferation. Slit2 also did not induce ERK1/2 and AKT1 phosphorylation in OVCAR-3 and SKOV-3 cells. The current findings indicate that three major members (Slit2/3 and Robo1) of Slit/Robo family are widely expressed in the human normal and malignant ovarian tissues and in OVCAR-3 and SKOV-3 cells. However, Slit/Robo signaling may not play an important role in regulating human ovarian cancer cell proliferation and migration.
Authors:
Cai Feng Dai; Yi Zhou Jiang; Yan Li; Kai Wang; Pei Shu Liu; Manish S Patankar; Jing Zheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-05
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  135     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-05     Completed Date:  2012-02-06     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  475-85     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Line, Tumor
Cell Movement
Cell Proliferation
Enzyme Activation
Extracellular Signal-Regulated MAP Kinases / metabolism
Female
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins / analysis,  biosynthesis,  metabolism*
Membrane Proteins / analysis,  biosynthesis,  metabolism*
Nerve Tissue Proteins / analysis,  biosynthesis,  metabolism*
Ovarian Neoplasms / metabolism*
Proto-Oncogene Proteins c-akt / metabolism
Receptors, Cell Surface / analysis,  biosynthesis,  metabolism*
Receptors, Immunologic / analysis,  biosynthesis,  metabolism*
Signal Transduction
Tissue Array Analysis
Grant Support
ID/Acronym/Agency:
HD38843/HD/NICHD NIH HHS; HL64703/HL/NHLBI NIH HHS; R01 HL064703/HL/NHLBI NIH HHS; R01 HL064703-08/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/ROBO4 protein, human; 0/Receptors, Cell Surface; 0/Receptors, Immunologic; 0/SLIT3 protein, human; 0/Slit homolog 2 protein; 0/roundabout protein; EC 2.7.11.1/AKT1 protein, human; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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