Document Detail


Expression and role of phosphodiesterase 3 in human squamous cell carcinoma KB cells.
MedLine Citation:
PMID:  12394274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphodiesterase (PDE) 3s have been characterized in human squamous cell carcinoma KB cells. PDE3 activity was detected in homogenates of KB cells. PDE3A and 3B mRNAs were detected by RT-PCR in RNA from KB cells; the nucleotide sequences of the fragments were identical to those of human PDE3A and 3B. Immunoblotting with anti-PDE3 antibodies detected both PDE3A- and 3B-immunoreactive proteins in KB cells. The PDE3-specific inhibitor, cilostamide, inhibited the proliferation of KB cells. Our results indicate that PDE3s may be important regulators of the growth of KB cells. Therefore, PDE3 inhibitors may be potential new drugs for antiproliferative therapies in squamous cell carcinoma in the head and neck.
Authors:
Kasumi Shimizu; Taku Murata; Kenya Okumura; Vincent C Manganiello; Toshiro Tagawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anti-cancer drugs     Volume:  13     ISSN:  0959-4973     ISO Abbreviation:  Anticancer Drugs     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-10-23     Completed Date:  2003-03-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9100823     Medline TA:  Anticancer Drugs     Country:  England    
Other Details:
Languages:  eng     Pagination:  875-80     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Mie 514-8507, Japan. skasumi@clin.medic.mie-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
3',5'-Cyclic-AMP Phosphodiesterases / genetics,  physiology*
Carcinoma, Squamous Cell / drug therapy
Cyclic Nucleotide Phosphodiesterases, Type 3
Humans
KB Cells / enzymology*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases; EC 3.1.4.17/Cyclic Nucleotide Phosphodiesterases, Type 3; EC 3.1.4.17/PDE3A protein, human

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