Document Detail


Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.
MedLine Citation:
PMID:  7514859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.
Authors:
T W North; G L Hansen; Y Zhu; J A Griffin; C K Shih
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  38     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-06-22     Completed Date:  1994-06-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  388-91     Citation Subset:  IM; X    
Affiliation:
Division of Biological Sciences, University of Montana, Missoula 59812.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Viral / genetics
Escherichia coli / enzymology*,  genetics*
Immunodeficiency Virus, Feline / enzymology*,  genetics*
Molecular Sequence Data
RNA-Directed DNA Polymerase / biosynthesis*,  genetics*,  isolation & purification
Recombinant Proteins / biosynthesis,  genetics,  isolation & purification
Virion / enzymology
Grant Support
ID/Acronym/Agency:
AI 28189/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Recombinant Proteins; EC 2.7.7.49/RNA-Directed DNA Polymerase
Comments/Corrections

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