| Expression of the retinoblastoma gene product in human hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 7535739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using the mouse anti-human retinoblastoma gene product (pRB) monoclonal antibody, PMG3-245, pRB was detected immunohistocytochemically in human hepatocellular carcinoma (HCC) tissues and a human HCC cell line, designated OCUH-16, recently established in our laboratory. This antibody reacted with human pRB and yielded a single band of approximately 110 kd from cultured OCUH-16 cells. The granules that stained for pRB were found mostly in the HCC cell nuclei, with a few granules observed in the rough endoplasmic reticulum by electron microscopy. Most of the stained granules were located in the euchromatin-rich areas. The percentage of OCUH-16 cells that expressed pRB or DNA polymerase alpha (DNA-PA) decreased over time as the number of OCUH-16 cells increased. The number of HCC cells that stained for pRB in the biopsy specimens from 11 patients varied and pRB expression was well maintained in early and advanced HCC. The level of pRB expression did not correlate with the differentiation of HCC cells or the clinical prognosis. The expression of pRB statistically correlated with that of DNA-PA (P < .01; r = .92). Some sinusoidal cells also stained for pRB. These findings imply that large deletions in the pRB gene are rare in the initiation or promotion of HCC. The correlation between pRB and DNA-PA may suggest that stained pRB participates in the proliferation of both HCC and non-HCC cells. |
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Authors:
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S Seki; N Kawakita; A Yanai; T Kitada; Y Sakai; K Nakatani; T Yamada; H Sakaguchi; T Kuroki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human pathology Volume: 26 ISSN: 0046-8177 ISO Abbreviation: Hum. Pathol. Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-05-09 Completed Date: 1995-05-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421547 Medline TA: Hum Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 366-74 Citation Subset: IM |
Affiliation:
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Third Department of Internal Medicine, Osaka City University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antibodies, Monoclonal Biopsy Blotting, Western Carcinoma, Hepatocellular / chemistry*, pathology Cell Nucleus / chemistry DNA Polymerase II / analysis Female Humans Liver Neoplasms / chemistry*, pathology Male Middle Aged Prognosis Retinoblastoma Protein / analysis* Staining and Labeling Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Retinoblastoma Protein; EC 2.7.7.-/DNA Polymerase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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