Document Detail

Expression, release, and biological activity of parathyroid hormone-related peptide from coronary endothelial cells.
MedLine Citation:
PMID:  10807866     Owner:  NLM     Status:  MEDLINE    
Ventricular cardiomyocytes have previously been identified as potential target cells for parathyroid hormone-related peptide (PTHrP). Synthetic PTHrP peptides exert a positive contractile effect. Because systemic PTHrP levels are normally negligible, this suggests that PTHrP is expressed in the ventricle and acts as a paracrine mediator. We investigated the ventricular expression of PTHrP and its expression in cultured cells isolated from the ventricle, studied the release of PTHrP from hearts and cultures, and investigated whether this authentic PTHrP mimics the biological effects previously described for synthetic PTHrP on ventricular cardiomyocytes. We found PTHrP expressed in ventricles of neonatal and adult rat hearts. In cells isolated from adult hearts, we found PTHrP expression exclusively in coronary endothelial cells but not in cardiomyocytes. The latter, however, are target cells for PTHrP. PTHrP was released from isolated perfused hearts during hypoxic perfusion and from cultured coronary endothelial cells under energy-depleting conditions. This PTHrP was biologically active; ie, it exerted a positive contractile and lusitropic effect on cardiomyocytes. Authentic PTHrP was glycosylated and showed a slightly higher potency than synthetic PTHrP. These results suggest that PTHrP is an endothelium-derived modulator of ventricular function.
K Schlüter; C Katzer; K Frischkopf; S Wenzel; G Taimor; H M Piper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation research     Volume:  86     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-14     Completed Date:  2000-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  946-51     Citation Subset:  IM    
Physiologisches Institut, Justus-Liebig-Universität Giessen, Giessen, Germany.
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MeSH Terms
Anoxia / metabolism
Cells, Cultured
Coronary Vessels / cytology,  drug effects,  metabolism*
Endothelium, Vascular / cytology,  metabolism*
Heart Ventricles
Myocardial Contraction / physiology
Myocardium / cytology,  metabolism
Parathyroid Hormone-Related Protein
Peptide Fragments / chemical synthesis,  pharmacology
Proteins / chemistry,  metabolism,  physiology*
Rats, Wistar
Tissue Distribution
Reg. No./Substance:
0/Parathyroid Hormone-Related Protein; 0/Peptide Fragments; 0/Proteins

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