Document Detail


Expression of regulatory genes during differentiation of thalamic nuclei in mouse and monkey.
MedLine Citation:
PMID:  15281080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression patterns of genes implicated in development of the thalamus were examined in mice and monkeys, using in situ hybridization with RNA probes complementary to Cad6, Dlx1, Dlx2, Dlx5, Gbx2, Id2, and Lef1 cDNAs. Expression patterns were related to the evolving cytoarchitecture in mice at birth (P0) and in adulthood, and in fetal monkeys early and late in the period of gestation when thalamic nuclei are becoming histologically differentiated out of a series of pronuclear masses. At the earlier developmental stage, each gene was expressed in a pattern that appeared to be pronucleus-specific and maintained a nucleus-specific pattern into adulthood, with the possible exception of Gbx2. Each gene displayed a unique expression pattern in the dorsal thalamus, ventral thalamus, and epithalamus, and no gene was expressed throughout all three divisions or in every nucleus of a division. With the exception of Dlx2, whose expression disappeared at the later time point, all continued to be expressed into adulthood at higher levels and with identical patterns. Despite late appearance of gamma-aminobutyric acid (GABA)ergic cells in the dorsal lateral geniculate nucleus of mice, no Dlx genes, which promote formation of a GABAergic phenotype elsewhere, were detected in dorsal thalamus. Each thalamic nucleus was distinguished by expression of a combination of genes, and homologous nuclei in mouse and monkey exhibited the same combination. The presence of a centre médian nucleus and four pulvinar nuclei in monkeys was marked by patterns of expression not found in mice. The centre médian nucleus was marked by high expression of Id2, which was expressed only weakly in very few nuclei of mice.
Authors:
Edward G Jones; John L R Rubenstein
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  477     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-07-28     Completed Date:  2004-09-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-80     Citation Subset:  IM    
Affiliation:
Center for Neuroscience, University of California, Davis, Davis, California 95616, USA. ejones@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cell Differentiation / physiology*
Cerebral Cortex / embryology,  growth & development,  metabolism
Embryo, Mammalian
Gene Expression Regulation, Developmental*
Genes, Regulator / physiology*
Haplorhini
In Situ Hybridization / methods
Mice
Mice, Inbred ICR
Neurons / metabolism
RNA, Complementary / metabolism
Species Specificity
Thalamic Nuclei / cytology,  embryology,  growth & development,  metabolism*
Grant Support
ID/Acronym/Agency:
MH01046/MH/NIMH NIH HHS; NS34661/NS/NINDS NIH HHS; NS39094/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Complementary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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