| Expression and regulation of tumor suppressor gene maspin in human bladder cancer. | |
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MedLine Citation:
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PMID: 14732229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Maspin is a member of serine protease inhibitor family with tumor suppressing activity for breast and prostate cancers, acting at the level of tumor invasion and metastasis. However, there have been no published data regarding the role of maspin in human bladder cancer. We evaluated maspin expression in 65 series of bladder cancer samples (22 transurethral resection (TUR) and 43 radical cystectomy) and studied the regulatory mechanism of maspin gene activation in bladder cancer cells. Maspin expression was immunohistochemically detected in four (18.2%) patients with TUR and 22 (51.2%) patients with radical cystectomy whereas no expression was observed in normal transitional cells located at tumor-free area in bladder. The maspin expression was significantly correlated with the development of muscle invasive bladder cancer (P=0.00008). Using a luciferase reporter system, maspin promoter activity was induced in the maspin-positive bladder cancer cell lines as well as maspin-negative RT4 cells. Furthermore, treatment with the DNA methyltransferase inhibitor, 5-aza-2' deoxycytidine, and histone deacetylase inhibitor, trichostatin A, led to re-expression of maspin in RT4 cells. Our results indicate that maspin may contribute to bladder cancer development and that DNA methylation and histone deacetylation may be important for regulating maspin gene activation in bladder cancer cells. |
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Authors:
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Shuji Sugimoto; Nicolai Maass; Yukie Takimoto; Katsuhiko Sato; Sadatsugu Minei; Ming Zhang; Yutaka Hoshikawa; Klaus-Peter Jünemann; Walter Jonat; Koichi Nagasaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer letters Volume: 203 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-20 Completed Date: 2004-03-10 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 209-15 Citation Subset: IM |
Affiliation:
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Department of Urology, University of Kiel, Arnold-Heller-strasse 7, Kiel 24105, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Azacitidine / analogs & derivatives*, pharmacology Carcinoma, Transitional Cell / metabolism Cell Line, Tumor Cytoplasm / metabolism DNA Methylation Female Gene Expression Regulation, Neoplastic* Genes, Reporter Genes, Tumor Suppressor Histones / metabolism Humans Hydroxamic Acids / pharmacology Immunohistochemistry Luciferases / metabolism Male Middle Aged Promoter Regions, Genetic Protein Biosynthesis* Proteins / genetics* Reverse Transcriptase Polymerase Chain Reaction Serpins / biosynthesis*, genetics* Transcriptional Activation Urinary Bladder Neoplasms / genetics, metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Histones; 0/Hydroxamic Acids; 0/Proteins; 0/SERPIN-B5; 0/Serpins; 2353-33-5/decitabine; 320-67-2/Azacitidine; 58880-19-6/trichostatin A; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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