Document Detail


Expression and regulation of tumor suppressor gene maspin in human bladder cancer.
MedLine Citation:
PMID:  14732229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maspin is a member of serine protease inhibitor family with tumor suppressing activity for breast and prostate cancers, acting at the level of tumor invasion and metastasis. However, there have been no published data regarding the role of maspin in human bladder cancer. We evaluated maspin expression in 65 series of bladder cancer samples (22 transurethral resection (TUR) and 43 radical cystectomy) and studied the regulatory mechanism of maspin gene activation in bladder cancer cells. Maspin expression was immunohistochemically detected in four (18.2%) patients with TUR and 22 (51.2%) patients with radical cystectomy whereas no expression was observed in normal transitional cells located at tumor-free area in bladder. The maspin expression was significantly correlated with the development of muscle invasive bladder cancer (P=0.00008). Using a luciferase reporter system, maspin promoter activity was induced in the maspin-positive bladder cancer cell lines as well as maspin-negative RT4 cells. Furthermore, treatment with the DNA methyltransferase inhibitor, 5-aza-2' deoxycytidine, and histone deacetylase inhibitor, trichostatin A, led to re-expression of maspin in RT4 cells. Our results indicate that maspin may contribute to bladder cancer development and that DNA methylation and histone deacetylation may be important for regulating maspin gene activation in bladder cancer cells.
Authors:
Shuji Sugimoto; Nicolai Maass; Yukie Takimoto; Katsuhiko Sato; Sadatsugu Minei; Ming Zhang; Yutaka Hoshikawa; Klaus-Peter Jünemann; Walter Jonat; Koichi Nagasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  203     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-20     Completed Date:  2004-03-10     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  209-15     Citation Subset:  IM    
Affiliation:
Department of Urology, University of Kiel, Arnold-Heller-strasse 7, Kiel 24105, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Azacitidine / analogs & derivatives*,  pharmacology
Carcinoma, Transitional Cell / metabolism
Cell Line, Tumor
Cytoplasm / metabolism
DNA Methylation
Female
Gene Expression Regulation, Neoplastic*
Genes, Reporter
Genes, Tumor Suppressor
Histones / metabolism
Humans
Hydroxamic Acids / pharmacology
Immunohistochemistry
Luciferases / metabolism
Male
Middle Aged
Promoter Regions, Genetic
Protein Biosynthesis*
Proteins / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Serpins / biosynthesis*,  genetics*
Transcriptional Activation
Urinary Bladder Neoplasms / genetics,  metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Histones; 0/Hydroxamic Acids; 0/Proteins; 0/SERPIN-B5; 0/Serpins; 320-67-2/Azacitidine; 3X2S926L3Z/trichostatin A; 776B62CQ27/decitabine; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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