Document Detail


Expression and regulation of the proenkephalin gene in rat Sertoli cells.
MedLine Citation:
PMID:  2464755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have shown that multiple forms of proenkephalin RNA are present in the rat and mouse testis that are cell type specific: a 1700-nucleotide transcript, unique to the testis, found in spermatogenic cells, and a 1450-nucleotide form of somatic cell origin. In the present studies, rat Sertoli cells are shown to contain the 1450-nucleotide form of proenkephalin RNA. The concentration of this transcript is quite low immediately after Sertoli cell isolation, but increases after 1 day in culture to a constant level. Proenkephalin RNA levels are stimulated severalfold by FSH and cAMP, but are not significantly affected by LH or testosterone. These studies demonstrate that Sertoli cells are at least one source of the somatic, 1450-nucleotide form of proenkephalin RNA in the rat testis. The expression of opioid peptide genes by Sertoli cells as well as by germ and Leydig cells is further evidence for the postulated autocrine or paracrine functions of these peptides in the testis.
Authors:
D Kew; D L Kilpatrick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  3     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-03-17     Completed Date:  1989-03-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
Affiliation:
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Animals
Cells, Cultured
Dihydrotestosterone / pharmacology
Enkephalins / genetics*
Follicle Stimulating Hormone / pharmacology
Forskolin / pharmacology
Gene Expression Regulation* / drug effects
Male
Protein Precursors / genetics*
RNA / analysis,  metabolism
Rats
Sertoli Cells / metabolism*
Testosterone / pharmacology
Time Factors
Grant Support
ID/Acronym/Agency:
DK36468/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Enkephalins; 0/Protein Precursors; 0/proenkephalin; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 28822-58-4/1-Methyl-3-isobutylxanthine; 521-18-6/Dihydrotestosterone; 58-22-0/Testosterone; 63231-63-0/RNA; 66428-89-5/Forskolin; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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