Document Detail

Expression and regulation of monocyte chemoattractant protein-1 by human eosinophils.
MedLine Citation:
PMID:  9130630     Owner:  NLM     Status:  MEDLINE    
Several recent studies have identified eosinophils as a cellular source of various cytokines, indicating that eosinophils play not only an effector role, but also a regulatory role within the allergic inflammatory cell network. In this study, we demonstrate that eosinophils can generate and secrete monocyte chemoattractant protein-1 (MCP-1), a prototype of C-C chemokines. Eosinophils generated immunoreactive MCP-1 in response to such diverse stimuli as C5a, formyl-methionyl-leucyl-phenylalanine (FMLP) and ionomycin, but MCP-1 production was not induced by interleukin (IL)-1 or tumor necrosis factor-alpha. C5a- and FMLP-induced eosinophil MCP-1 production was absolutely dependent on pretreatment with cytochalasin B. Eosinophils elaborated significantly more MCP-1 than neutrophils. Immunoreactive MCP-1 was detected at 6 h of incubation with C5a or FMLP. Expression of MCP-1 mRNA reached a maximum within the first 3 h after stimulation and then declined rapidly to a very low and stable level by 18 h. Pretreatment with IL-5 markedly amplified C5a-induced MCP-1 production, and the enhancement occurred at the pretranslational level. Eosinophil-active chemokines such as eotaxin failed to induce MCP-1 generation, even when eosinophils were primed by IL-5. Since MCP-1 exerts a potent histamine-releasing effect on human basophils, our results indicate that eosinophils may regulate basophil mediator release with possible consequent contribution to the pathogenesis of allergic inflammation via a paracrine mechanism.
S Izumi; K Hirai; M Miyamasu; Y Takahashi; Y Misaki; T Takaishi; Y Morita; K Matsushima; N Ida; H Nakamura; T Kasahara; K Ito
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  27     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-22     Completed Date:  1997-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  816-24     Citation Subset:  IM    
Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.
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MeSH Terms
Chemokine CCL2 / biosynthesis*,  genetics,  immunology
Chemokines / pharmacology
Complement C5a / agonists,  pharmacology
Eosinophils / chemistry,  drug effects,  immunology*,  metabolism*
Interleukin-5 / agonists,  pharmacology
Monocytes / drug effects,  metabolism
Neutrophils / drug effects,  metabolism
RNA, Messenger / biosynthesis
Reg. No./Substance:
0/Chemokine CCL2; 0/Chemokines; 0/Interleukin-5; 0/RNA, Messenger; 80295-54-1/Complement C5a

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