| Expression and regulation of monocyte chemoattractant protein-1 by human eosinophils. | |
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MedLine Citation:
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PMID: 9130630 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several recent studies have identified eosinophils as a cellular source of various cytokines, indicating that eosinophils play not only an effector role, but also a regulatory role within the allergic inflammatory cell network. In this study, we demonstrate that eosinophils can generate and secrete monocyte chemoattractant protein-1 (MCP-1), a prototype of C-C chemokines. Eosinophils generated immunoreactive MCP-1 in response to such diverse stimuli as C5a, formyl-methionyl-leucyl-phenylalanine (FMLP) and ionomycin, but MCP-1 production was not induced by interleukin (IL)-1 or tumor necrosis factor-alpha. C5a- and FMLP-induced eosinophil MCP-1 production was absolutely dependent on pretreatment with cytochalasin B. Eosinophils elaborated significantly more MCP-1 than neutrophils. Immunoreactive MCP-1 was detected at 6 h of incubation with C5a or FMLP. Expression of MCP-1 mRNA reached a maximum within the first 3 h after stimulation and then declined rapidly to a very low and stable level by 18 h. Pretreatment with IL-5 markedly amplified C5a-induced MCP-1 production, and the enhancement occurred at the pretranslational level. Eosinophil-active chemokines such as eotaxin failed to induce MCP-1 generation, even when eosinophils were primed by IL-5. Since MCP-1 exerts a potent histamine-releasing effect on human basophils, our results indicate that eosinophils may regulate basophil mediator release with possible consequent contribution to the pathogenesis of allergic inflammation via a paracrine mechanism. |
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Authors:
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S Izumi; K Hirai; M Miyamasu; Y Takahashi; Y Misaki; T Takaishi; Y Morita; K Matsushima; N Ida; H Nakamura; T Kasahara; K Ito |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of immunology Volume: 27 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-05-22 Completed Date: 1997-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 816-24 Citation Subset: IM |
Affiliation:
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Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chemokine CCL2
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biosynthesis*,
genetics,
immunology Chemokines / pharmacology Complement C5a / agonists, pharmacology Eosinophils / chemistry, drug effects, immunology*, metabolism* Humans Immunohistochemistry Interleukin-5 / agonists, pharmacology Kinetics Monocytes / drug effects, metabolism Neutrophils / drug effects, metabolism RNA, Messenger / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Chemokines; 0/Interleukin-5; 0/RNA, Messenger; 80295-54-1/Complement C5a |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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