Document Detail


Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.
MedLine Citation:
PMID:  10872333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A plasmid including a mouse immunoglobulin mu gene was transfected into the IgG-secreting human lymphoid line HMy2 and mouse B- and pre-B-cell lines WEHI 231 and 18-81; stably transfected cells were selected. Transfected HMy2 cells synthesized mouse immunoglobulin mu chains as a major secreted protein but the WEHI 231 and 18-81 transfectants transcribed the introduced mu gene at lower levels. In HMy2 transfectants, most of the transcription of the introduced heavy chain gene initiated 40 and 62 bp upstream of the beginning of the VH exon translation start, although a small proportion of transcripts initiating further upstream was detected. WEHI 231 and 18-81 transfectants gave a much higher proportion of upstream initiation. Transient expression of the VH exon was monitored following transfection of mouse myeloma with the VH gene DNA in various plasmid constructs. VH transcription was only observed if the plasmids contained a segment derived from the large VH-CH intron of the immunoglobulin heavy chain locus. This segment, located between JH and switch regions, functioned both downstream of the VH exon and upstream in either orientation. The existence of a transcription enhancer element in this region is therefore proposed.
Authors:
M S Neuberger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The EMBO journal     Volume:  2     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1983  
Date Detail:
Created Date:  2000-07-24     Completed Date:  2000-07-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1373-8     Citation Subset:  IM    
Affiliation:
MRC Laboratory of Molecular Biology, Cambridge, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites
Cell Line
Gene Expression Regulation*
Genes, Immunoglobulin*
Humans
Immunoglobulin Joining Region / genetics*
Immunoglobulin Variable Region / genetics*
Immunoglobulin mu-Chains / genetics*
Lymphocytes / cytology
Mice
Molecular Sequence Data
Plasmids
Transcription, Genetic
Transfection
Chemical
Reg. No./Substance:
0/Immunoglobulin Joining Region; 0/Immunoglobulin Variable Region; 0/Immunoglobulin mu-Chains
Comments/Corrections

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