Document Detail


Expression, regulation and functional characterization of matrix metalloproteinase-3 of human trophoblast.
MedLine Citation:
PMID:  19155066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MMP-3 has been detected in human placenta and reduced expression of the enzyme was observed in invasive trophoblasts of patients with severe preeclampsia. However, detailed expression pattern, regulation and biological properties of the placental protease have not been elucidated so far. RT-PCR analyses, Western blotting and enzyme activity assays revealed that pro- and active form of MMP-3 were predominantly expressed in purified first trimester villous trophoblasts, in invasive cytotrophoblasts of differentiating explant cultures and in trophoblastic SGHPL-4 cells. Accordingly, immunofluorescene of first trimester placental tissues detected MMP-3 mainly in villous and extravillous cytotrophoblasts. IL-1beta, an inducer of MMP-3 in decidual cells, increased secretion and activity of the protease in trophoblast supernatants in a dose- and time-dependent manner. IL-1beta-stimulated production of the enzyme was suppressed in the presence of inhibitors of MAPK and AKT signalling. Similar to recombinant MMP-3, MMP-3 in supernatants of IL-1beta-stimulated decidual stromal or SGHPL-4 cells degraded IGFBP-1 in vitro resulting in the appearance of cleavage products at approximately 25, 22, 17, 14 and 11kD. However, cleavage assays using recombinant MMP-2 suggested that the gelatinase may contribute to IGFBP-1 degradation in trophoblast supernatants. Despite its effects on MMP-3 expression IL-1beta failed to significantly alter invasion of SGHPL-4 cells through Matrigel-coated transwells. In conclusion, the data suggest that invasive trophoblast cell models secrete bioactive MMP-3. Inducible expression of the protease involves MAPK and AKT signalling. In addition to the decidua, MMP-3 of trophoblasts may contribute to the regulation of the IGF system by degrading IGFBP-1.
Authors:
H Husslein; S Haider; G Meinhardt; J Prast; S Sonderegger; M Knöfler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-19
Journal Detail:
Title:  Placenta     Volume:  30     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-19     Completed Date:  2009-05-14     Revised Date:  2013-02-26    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  284-91     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Fetal-Maternal Medicine, Reproductive Biology Unit, Medical University of Vienna, Waehringer Guertel 18-20, Vienna A-1090, Austria.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Female
Fibroblasts / metabolism
Humans
Insulin-Like Growth Factor Binding Protein 1 / metabolism*
Interleukin-1beta / metabolism
MAP Kinase Signaling System
Matrix Metalloproteinase 3 / metabolism*
Placenta / metabolism*
Pregnancy
Proto-Oncogene Proteins c-akt / metabolism
Trophoblasts / metabolism*
Grant Support
ID/Acronym/Agency:
P 17894-B14//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
0/IGFBP1 protein, human; 0/Insulin-Like Growth Factor Binding Protein 1; 0/Interleukin-1beta; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.4.24.17/MMP3 protein, human; EC 3.4.24.17/Matrix Metalloproteinase 3
Comments/Corrections

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