Document Detail

Expression and regulation of collagenase-2 (MMP-8) in head and neck squamous cell carcinomas.
MedLine Citation:
PMID:  12081207     Owner:  NLM     Status:  MEDLINE    
MMP-8 (collagenase-2) is the most effective collagenase to initiate type I collagen degradation. Since initiation of lysis of the surrounding collagen matrix is an essential prerequisite for carcinoma cells to spread, this study investigated the expression of MMP-8 in squamous cell carcinoma (SCC) of the head and neck in vivo and in vitro. Most of the recently established head and neck carcinoma cell lines (22/25), corresponding tumour (5/7) and dermal (2/2) fibroblasts, commercial tongue carcinoma (HSC-3 and SCC-25), and transformed keratinocyte cell lines of the tongue (IHGK) and skin (HaCaT) expressed MMP-8 mRNA analysed by the PCR method. Western blotting revealed a latent 50 kD band in concentrated culture media of carcinoma cells and corresponding tumour and dermal fibroblasts. The expression of immunoreactive MMP-8 protein was reduced 30% by transforming growth factor beta-1 (TGF-beta1) at 1 ng/ml concentration and 60% at 10 ng/ml concentration, but up-regulated 2- and 2.5-fold after 10 nM and 100 nM phorbol 12-myristate 13 acetate (PMA), respectively. Immunohistological staining localized MMP-8 protein in a few malignant invading tumour cell islands, certain fibroblasts, polymorphonuclear neutrophils (PMNs), and plasma cells. In situ hybridization revealed a faint sporadic signal in carcinoma cells of all eight tissue sections analysed. It is concluded that tissue from head and neck carcinomas can express MMP-8 both in vivo and in vitro. Since the amount of MMP-8 in carcinoma and stromal cells is rather low, MMP-8 may have a potential role, with other collagenases, in the proteolysis of connective tissue associated with the spreading of invasive carcinoma.
M Moilanen; E Pirilä; R Grénman; T Sorsa; T Salo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  197     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-25     Completed Date:  2002-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  72-81     Citation Subset:  IM    
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Finland.
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MeSH Terms
Blotting, Southern
Blotting, Western
Carcinoma, Squamous Cell / enzymology*,  genetics
Gene Expression Regulation, Neoplastic / drug effects
Head and Neck Neoplasms / enzymology*,  genetics
In Situ Hybridization
Matrix Metalloproteinase 8 / genetics,  metabolism*
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Tetradecanoylphorbol Acetate / pharmacology
Transforming Growth Factor beta / pharmacology
Transforming Growth Factor beta1
Tumor Cells, Cultured
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Neoplasm; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 16561-29-8/Tetradecanoylphorbol Acetate; EC Metalloproteinase 8

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