| Expression and regulation of collagenase-2 (MMP-8) in head and neck squamous cell carcinomas. | |
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MedLine Citation:
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PMID: 12081207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MMP-8 (collagenase-2) is the most effective collagenase to initiate type I collagen degradation. Since initiation of lysis of the surrounding collagen matrix is an essential prerequisite for carcinoma cells to spread, this study investigated the expression of MMP-8 in squamous cell carcinoma (SCC) of the head and neck in vivo and in vitro. Most of the recently established head and neck carcinoma cell lines (22/25), corresponding tumour (5/7) and dermal (2/2) fibroblasts, commercial tongue carcinoma (HSC-3 and SCC-25), and transformed keratinocyte cell lines of the tongue (IHGK) and skin (HaCaT) expressed MMP-8 mRNA analysed by the PCR method. Western blotting revealed a latent 50 kD band in concentrated culture media of carcinoma cells and corresponding tumour and dermal fibroblasts. The expression of immunoreactive MMP-8 protein was reduced 30% by transforming growth factor beta-1 (TGF-beta1) at 1 ng/ml concentration and 60% at 10 ng/ml concentration, but up-regulated 2- and 2.5-fold after 10 nM and 100 nM phorbol 12-myristate 13 acetate (PMA), respectively. Immunohistological staining localized MMP-8 protein in a few malignant invading tumour cell islands, certain fibroblasts, polymorphonuclear neutrophils (PMNs), and plasma cells. In situ hybridization revealed a faint sporadic signal in carcinoma cells of all eight tissue sections analysed. It is concluded that tissue from head and neck carcinomas can express MMP-8 both in vivo and in vitro. Since the amount of MMP-8 in carcinoma and stromal cells is rather low, MMP-8 may have a potential role, with other collagenases, in the proteolysis of connective tissue associated with the spreading of invasive carcinoma. |
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Authors:
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M Moilanen; E Pirilä; R Grénman; T Sorsa; T Salo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pathology Volume: 197 ISSN: 0022-3417 ISO Abbreviation: J. Pathol. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-06-25 Completed Date: 2002-07-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0204634 Medline TA: J Pathol Country: England |
Other Details:
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Languages: eng Pagination: 72-81 Citation Subset: IM |
Affiliation:
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Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Southern Blotting, Western Carcinoma, Squamous Cell / enzymology*, genetics Female Gene Expression Regulation, Neoplastic / drug effects Head and Neck Neoplasms / enzymology*, genetics Humans In Situ Hybridization Male Matrix Metalloproteinase 8 / genetics, metabolism* RNA, Messenger / genetics RNA, Neoplasm / genetics Tetradecanoylphorbol Acetate / pharmacology Transforming Growth Factor beta / pharmacology Transforming Growth Factor beta1 Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Neoplasm; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 16561-29-8/Tetradecanoylphorbol Acetate; EC 3.4.24.34/Matrix Metalloproteinase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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