Document Detail


Expression of recombinant human nerve growth factor in Escherichia coli.
MedLine Citation:
PMID:  1304315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nerve growth factor (NGF) is a neurotrophic factor for basal forebrain cholinergic neurons and may be of benefit in neurodegenerative diseases of humans. A method is described to obtain significant amounts of biologically active recombinant human NGF (rhNGF) in one step. RhNGF was expressed in E. coli and the majority of the protein accumulated in inclusion bodies. It was immunoprecipitated by a serum against mouse NGF. Solubilization of the inclusion bodies was done in 3M guanidine HCl and renaturation was effected by dilution and air oxidation in the presence of 6 microM CuSO4. Recoveries were 10-12 micrograms of rhNGF per ml of bacterial suspension. Its biological activity was tested in a bioassay system employing sympathetic chick embryo ganglia and was inhibited by the monoclonal antibody 27/21 against mouse NGF.
Authors:
E Dicou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemistry international     Volume:  20     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1993-07-07     Completed Date:  1993-07-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  129-34     Citation Subset:  IM    
Affiliation:
INSERM U 298, Centre Hospitalier Régional et Universitaire, Angers, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Base Sequence
Biological Assay
Chick Embryo
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics
Ganglia, Sympathetic / drug effects
Humans
Molecular Sequence Data
Nerve Growth Factors / biosynthesis*,  pharmacology
Oligodeoxyribonucleotides
Plasmids
Promoter Regions, Genetic
Recombinant Proteins / biosynthesis*,  isolation & purification,  pharmacology
Restriction Mapping
Terminator Regions, Genetic
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Nerve Growth Factors; 0/Oligodeoxyribonucleotides; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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