Document Detail


Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC.
MedLine Citation:
PMID:  18406177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To express recombinant proteins in the spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) currently established in our laboratory, a chemically synthesized DNA fragment encoding the anticoagulant hirudin was used to construct a mammalian expression vector under the control of the goat beta-casein regulatory sequence. The vector, named pGB562/Hi, was transfected into the SI-PMEC cells to yield pGB562/Hi/SI-PMEC. The pGB562/Hi/SI-PMEC cells expressed recombinant hirudin only when they were differentiated into functional structures by growth on a Matrigel-coated petri dish supplemented with the lactogenic hormone prolactin. The differentiated pGB562/Hi/SI-PMEC cells produced about 0.5-0.6microg of recombinant hirudin/mg of total cellular protein. These results suggest that the established SI-PMEC cells have pharmaceutical potential to inducibly express bioactive heterogeneous proteins.
Authors:
Y L Sun; Y C Chou; T C Kuan; C F Tu; C S Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-02     Completed Date:  2008-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  739-47     Citation Subset:  IM    
Affiliation:
Division of Biotechnology, Animal Technology Institute, Taiwan, P.O. Box 23, Chunan 35099, Miaoli, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Collagen / pharmacology
Drug Combinations
Epithelial Cells / cytology,  metabolism*
Fibrinolytic Agents / metabolism
Gene Expression
Genetic Vectors
Hirudins / biosynthesis*,  genetics
Laminin / pharmacology
Mammary Glands, Animal
Molecular Sequence Data
Prolactin / pharmacology
Proteoglycans / pharmacology
Recombinant Proteins / biosynthesis,  genetics
Swine
Transfection
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Fibrinolytic Agents; 0/Hirudins; 0/Laminin; 0/Proteoglycans; 0/Recombinant Proteins; 119978-18-6/matrigel; 9002-62-4/Prolactin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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