Document Detail


Expression rate of vinculin isoforms in human aortocoronary saphenous vein grafts.
MedLine Citation:
PMID:  9158163     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aortocoronary bypass conduits derived from saphenous veins usually develop diffuse intimal thickening, one of the major causes of haemodynamically relevant graft stenosis. To elucidate the role of smooth muscle cell proliferation in late graft failure, specimens from highly stenotic or occluded vein grafts implanted into the arterial circulation for more than 5 years were tested for their expression rate of meta-vinculin. Since the cytoskeletal protein meta-vinculin is present exclusively in contractile smooth muscle cells, the determination of the relative amounts of meta-vinculin (150 kDa) and its low-molecular weight isoform vinculin (130 kDa) allows characterization of the phenotypic status of smooth muscle cells. Using immunoblotting techniques, the quantitative relation of meta-vinculin in tissue samples obtained from autoptic vein grafts (n=10) was measured and compared with those of native saphenous veins (n=6). In vein grafts, the fractional meta-vinculin content of the total vinculin immunoreactivity ranged from 32%-46% (mean 39.6%), whereas the range was 39%-53% (mean 46.7%) in native veins. By applying Student's t-test, a statistical significance was not demonstrated suggesting that the majority of smooth muscle cells in intimal thickenings consisted of a contractile phenotype. Immunohistochemically, the vinculin immunoreactivity in the intimal layer of vein grafts was reduced as compared to native saphenous veins. The distribution of vinculin in grafted veins closely resembled that in arteriosclerotic coronary arteries with intimal thickening. Hence, our biochemical data demonstrate parallels between the pathogenesis of late vein graft stenosis and degenerative arteriosclerotic lesions.
Authors:
U Brinck; M Mirzaie; M Korabiowska; T Meyer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of cardiology     Volume:  59     ISSN:  0167-5273     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-06-10     Completed Date:  1997-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  125-32     Citation Subset:  IM    
Affiliation:
Institute of Pathology, University of Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actins / analysis
Calcium-Binding Proteins / analysis
Cell Division
Constriction, Pathologic / metabolism,  pathology
Coronary Artery Bypass*
Coronary Artery Disease / metabolism,  pathology
Gene Expression Regulation
Graft Occlusion, Vascular / metabolism,  pathology
Humans
Hyperplasia
Immunoblotting
Immunohistochemistry
Membrane Proteins / analysis,  genetics
Microfilament Proteins / analysis
Molecular Weight
Muscle Proteins / analysis,  genetics
Muscle, Smooth, Vascular / metabolism,  pathology
Phenotype
Saphenous Vein / metabolism,  pathology,  transplantation*
Tunica Intima / metabolism,  pathology
Vinculin / analysis*,  classification,  genetics
Chemical
Reg. No./Substance:
0/Actins; 0/Calcium-Binding Proteins; 0/Membrane Proteins; 0/Microfilament Proteins; 0/Muscle Proteins; 0/VCL protein, human; 0/calponin; 125361-02-6/Vinculin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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