| Expression of rat brain excitatory amino acid receptors in Xenopus oocytes. | |
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MedLine Citation:
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PMID: 2449049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xenopus laevis oocytes when injected with rat brain mRNA synthesize neuronal receptors that can be analyzed electrophysiologically. After a post-injection incubation period of 24-72 hours, L-glutamic acid, kainic acid and quisqualic acid caused a dose dependent (10-100 microM) depolarization of the oocyte membrane. The voltage and conductance changes associated with kainate activation were distinguishable from those seen for L-glutamate or quisqualate. There was no response to L-aspartate application and an inconsistent response to N-methyl-D-aspartate. Upon fractionation of the mRNA on sucrose gradients, transcripts greater than 2 Kb in length were obligatory for the synthesis of excitatory amino acid receptors. The electrophysiological response of injected oocytes exposed to L-glutamate was similar to that of native oocytes when exposed to muscarinic agents. This similarity may reflect the activation of the same ionophore and suggests that the active mRNA fraction for glutamate responsiveness either encodes for a binding protein that can be assembled along with native ion channels into the oocyte membrane or encodes for a glutamate binding site with a similar channel. |
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Authors:
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R A Lampe; L G Davis; M J Gutnick |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Advances in experimental medicine and biology Volume: 221 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 1987 |
Date Detail:
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Created Date: 1988-02-29 Completed Date: 1988-02-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 201-10 Citation Subset: IM |
Affiliation:
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Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, DE 19898. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology* Animals Brain / metabolism* Cells, Cultured Female Oocytes / drug effects, metabolism*, physiology Poly A / genetics Protein Biosynthesis* RNA / genetics RNA, Messenger / genetics Rats Receptors, Cell Surface / drug effects, genetics* Transcription, Genetic* Xenopus laevis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/RNA, Messenger; 0/Receptors, Cell Surface; 24937-83-5/Poly A; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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