| Expression of the rabbit sperm protein Sp17 in COS cells and interaction of recombinant Sp17 with the rabbit zona pellucida. | |
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MedLine Citation:
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PMID: 7702869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the crosslinking, reagent DSS that rSp17 can bind to rabbit zona glycoprotein R45 or R55. |
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Authors:
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N Yamasaki; R T Richardson; M G O'Rand |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Molecular reproduction and development Volume: 40 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-05-10 Completed Date: 1995-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 48-55 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantigens / genetics, metabolism Base Sequence Carrier Proteins / genetics*, immunology, metabolism* Cell Line DNA Primers / genetics Egg Proteins / metabolism Female Gene Expression Male Membrane Glycoproteins / metabolism Microscopy, Fluorescence Microscopy, Phase-Contrast Molecular Sequence Data Protein Binding Rabbits Receptors, Cell Surface* Recombinant Proteins / genetics, immunology, metabolism Transfection Zona Pellucida / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/Carrier Proteins; 0/DNA Primers; 0/Egg Proteins; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Recombinant Proteins; 0/zona pellucida glycoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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