Document Detail


Expression of the rabbit sperm protein Sp17 in COS cells and interaction of recombinant Sp17 with the rabbit zona pellucida.
MedLine Citation:
PMID:  7702869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the crosslinking, reagent DSS that rSp17 can bind to rabbit zona glycoprotein R45 or R55.
Authors:
N Yamasaki; R T Richardson; M G O'Rand
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  40     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-10     Completed Date:  1995-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  48-55     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantigens / genetics,  metabolism
Base Sequence
Carrier Proteins / genetics*,  immunology,  metabolism*
Cell Line
DNA Primers / genetics
Egg Proteins / metabolism
Female
Gene Expression
Male
Membrane Glycoproteins / metabolism
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Molecular Sequence Data
Protein Binding
Rabbits
Receptors, Cell Surface*
Recombinant Proteins / genetics,  immunology,  metabolism
Transfection
Zona Pellucida / metabolism*
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Carrier Proteins; 0/DNA Primers; 0/Egg Proteins; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Recombinant Proteins; 0/zona pellucida glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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