| Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98. | |
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MedLine Citation:
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PMID: 20054138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Maleylacetate reductase (EC 1.3.1.32) is an important enzyme that is involved in the degradation pathway of aromatic compounds and catalyzes the reduction of maleylacetate to 3-oxoadipate. The gene pnpD encoding maleylacetate reductase in Burkholderia sp. strain SJ98 was cloned, expressed in Escherichia coli and purified by affinity chromatography. The enzyme was crystallized in both native and SeMet-derivative forms by the sitting-drop vapour-diffusion method using PEG 3350 as a precipitant at 293 K. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 72.91, b = 85.94, c = 53.07 A. X-ray diffraction data for the native and SeMet-derivative crystal were collected to 2.7 and 2.9 A resolution, respectively. |
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Authors:
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Archana Chauhan; Zeyaul Islam; Rakesh Kumar Jain; Subramanian Karthikeyan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-27 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 65 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-07 Completed Date: 2010-03-19 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 1313-6 Citation Subset: IM |
Affiliation:
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Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160 036, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Burkholderia / enzymology*, genetics Crystallization Crystallography, X-Ray DNA Primers / genetics Genes, Bacterial Oxidoreductases Acting on CH-CH Group Donors / chemistry*, genetics, isolation & purification Protein Conformation Recombinant Proteins / chemistry, genetics, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Recombinant Proteins; EC 1.3.-/Oxidoreductases Acting on CH-CH Group Donors; EC 1.3.1.32/maleylacetate reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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