| Expression, purification, and characterization of recombinant Saccharomyces cerevisiae adenosine kinase. | |
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MedLine Citation:
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PMID: 12883639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenosine kinase (AK), a key enzyme in the regulation of the cellular concentrations of adenosine (A), is an important physiological effector of many cells and tissues. In this article, we reported that ak, which encoded adenosine kinase, was cloned from Saccharomyces cerevisiae, sequenced, and overexpressed in E. coli using the pET16b expression system, and the recombinant protein was purified to apparent homogeneity using conventional protein purification techniques. Kinetic analysis of S. cerevisiae AK revealed K(m) values of (3.5+/-0.2) micromol/L for adenosine and (100.0+/-11.0) micromol/L for ATP, with k(cat) of (1530+/-20) min(-1) for adenosine and (1448+/-25) min(-1) for ATP. The determination of the K(m) value for other nucleosides and deoxynucleoside indicated that the nucleoside specificity of this enzyme from yeast was quite high. |
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Authors:
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Xiao-Bing Lu; Hai-Zhen Wu; Jiang Ye; Yi Fan; Hui-Zhan Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Volume: 35 ISSN: 0582-9879 ISO Abbreviation: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-07-28 Completed Date: 2003-12-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 20730160R Medline TA: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 666-70 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. huizhzh@online.sh.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Kinase
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genetics*,
isolation & purification,
metabolism Escherichia coli / genetics Kinetics Recombinant Proteins / biosynthesis, isolation & purification Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae Proteins / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.1.20/Adenosine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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