Document Detail


Expression of the protein product of the prostaglandin synthase-2/TIS10 gene in mitogen-stimulated Swiss 3T3 cells.
MedLine Citation:
PMID:  8449903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TIS10/PGS-2 encodes a prostaglandin synthase (PGS) distinct from the previously described enzyme PGS-1 (EC 1.14.99.1). We have now generated antipeptide antisera, directed to an amino acid sequence unique to the murine TIS10/PGS-2 protein, which specifically recognize the TIS10/PGS-2 antigen. TIS10/PGS-2 protein was undetectable in quiescent Swiss 3T3 cells. The level of TIS10/PGS-2 protein peaked between 6 and 8 h following phorbol ester stimulation of cells, then declined to basal levels after 18-24 h. Synthesis of TIS10/PGS-2 protein was dramatically increased in the second hour following mitogen stimulation and remained elevated for several hours. The half-life of the TIS10/PGS-2 protein was 4 h. Immunofluorescence studies demonstrated a perinuclear and cytoplasmic localization of the TIS10/PGS-2 antigen. As expected, detection of induced TIS10/PGS-2 antigen was dependent on protein synthesis. Metabolically labeled TIS10/PGS-2 protein migrated as a 71/73-kDa doublet following immunoprecipitation. Dexamethasone blocked both the TPA- and serum-induced appearance of TIS10/PGS-2 antigen. These studies demonstrate the existence of a mitogen-inducible, glucocorticoid-inhibitable, immunologically distinct prostaglandin synthase protein.
Authors:
D A Kujubu; S T Reddy; B S Fletcher; H R Herschman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  268     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-15     Completed Date:  1993-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5425-30     Citation Subset:  IM    
Affiliation:
Division of Nephrology, UCLA School of Medicine 90024.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Amino Acid Sequence
Animals
Cell Line, Transformed
Cercopithecus aethiops
Cyclooxygenase Inhibitors / pharmacology
Dexamethasone / pharmacology
Enzyme Induction
Fluorescent Antibody Technique
Mice
Mitogens
Molecular Sequence Data
Prostaglandin-Endoperoxide Synthases / biosynthesis*,  genetics,  immunology
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
ID/Acronym/Agency:
1K11 01815//PHS HHS; M24797//PHS HHS
Chemical
Reg. No./Substance:
0/Cyclooxygenase Inhibitors; 0/Mitogens; 16561-29-8/Tetradecanoylphorbol Acetate; 50-02-2/Dexamethasone; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

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