Document Detail

Expression of protamine P2 in the testis of the common marmoset and man visualized using non-radioactive in-situ hybridization.
MedLine Citation:
PMID:  8940659     Owner:  NLM     Status:  MEDLINE    
Information on the organization of the spermatogenic cycle of the common marmoset (Callíthrix jacchus), a small New World primate, is limited to a single histological report on the differentiation of spermatids. In the present study we have used non-radioactive in-situ hybridization with a cRNA probe directed against marmoset protamine 2, on fixed sections of marmoset and human testis to elucidate the organization of mature germ cells within the seminiferous epithelium. Specificity of the probe was checked on Northern blots; mP2 hybridized exclusively to mRNA in samples extracted from marmoset and human testis. In sections from human and marmoset testis, positive staining for mRNA was confined to round and elongating spermatids and in the human was reduced in samples from patients with incomplete spermatogenesis. In the human, P2 mRNA was present in groups of cells consistent with the presence of more than one stage of the spermatogenic cycle in transverse sections of individual tubules. In the marmoset, P2-positive cells were detected as a continuous ring of staining in the majority of sections of tubules whilst in others only a group(s) of cells was positive. We conclude that the arrangement of the spermatogenic wave in this New World primate may be intermediate between that seen in rodents (segmental) and in the human (helical).
P T Saunders; J Gaughan; B A Saxty; L E Kerr; M R Millar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of andrology     Volume:  19     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-02-27     Completed Date:  1997-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  212-9     Citation Subset:  IM    
MRC Reproductive Biology Unit, Edinburgh, UK.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Callithrix / genetics,  metabolism
Gene Expression*
In Situ Hybridization
Isotope Labeling
Molecular Sequence Data
Protamines / genetics*
RNA, Messenger
Sequence Analysis, DNA
Testis / metabolism*,  ultrastructure
Reg. No./Substance:
0/Protamines; 0/RNA, Messenger; 0/protamine P2

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