Document Detail


Expression of prostaglandin transporter in the bovine uterus and fetal membranes during pregnancy.
MedLine Citation:
PMID:  15814899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uteroplacental prostaglandins (PGs) play pivotal roles in the maintenance and termination of pregnancy in mammals. In the present study, we have characterized the expression of prostaglandin transporter (PGT) in placentome caruncles, intercaruncular tissues, fetal membranes, and utero-ovarian plexus during pregnancy in cattle. Pregnant bovine uteri were collected and classified into six groups covering the entire gestational length. In caruncles and intercaruncular tissues, PGT mRNA (also known as SLC02A1) and PGT protein were highly expressed at the late stage of pregnancy compared to the early and mid stages, whereas the level of expression is constant and low in fetal membranes throughout pregnancy. PGT mRNA and PGT protein were expressed at a constant level in the utero-ovarian plexus both ipsilateral and contralateral to corpus luteum throughout the course of pregnancy. Overall, the relative expression of PGT mRNA and PGT protein were higher in caruncles than in intercaruncular tissue and fetal membranes, whereas no differences were detected between intercaruncular tissues and fetal membranes at any stage of gestation. Immunohistochemistry indicated that PGT was preferentially expressed in caruncular epithelial cells of placentomes and endometrial luminal epithelial and myometrial smooth muscle cells of the intercaruncular regions. The level of PGT expression was comparatively higher in maternal components than in fetal components. In conclusion, differential spatiotemporal tissue-specific expression of PGT in uterine and intrauterine tissues suggests a role for this transporter in the exchange of PGs between the maternal and the fetal compartments, as well as for intrauterine metabolism of PGs during pregnancy.
Authors:
S K Banu; J A Arosh; P Chapdelaine; M A Fortier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-06
Journal Detail:
Title:  Biology of reproduction     Volume:  73     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-21     Completed Date:  2005-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  230-6     Citation Subset:  IM    
Affiliation:
Unité d'Ontogénie et Reproduction, Centre Hospitalier Universitaire de Québec, Université Laval, Québec, Canada G1K 7P4.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiporters / biosynthesis*,  genetics
Blotting, Northern / veterinary
Blotting, Western / veterinary
Cattle / metabolism*
DNA-Binding Proteins / biosynthesis*,  genetics
Extraembryonic Membranes / metabolism*
Female
Fetus
Immunohistochemistry / veterinary
Organic Anion Transporters
Placenta / metabolism*
Pregnancy
Pregnancy, Animal / metabolism*
RNA, Messenger / biosynthesis,  genetics
Uterus / metabolism*
Chemical
Reg. No./Substance:
0/Antiporters; 0/DNA-Binding Proteins; 0/Organic Anion Transporters; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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