| Expression and properties of feline herpesvirus type 1 gD (hemagglutinin) by a recombinant baculovirus. | |
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MedLine Citation:
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PMID: 9029779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We constructed a recombinant baculovirus expressing feline herpesvirus type I (FHV-1) gD in insect cells (Sf9 cells). The expressed product was identified as FHV-1 gD by a panel of monoclonal antibodies specific for the FHV-1 gD, and had an apparent molecular mass of approximately 49 kDa, which was less than that of the authentic FHV-1 gD. When the FHV-1 gD protein were expressed in Sf9 cells and CRFK cells in the presence of tunicamycin, the FHV-1 gD exhibited a molecular mass of 41 kDa. It was shown that the gD protein was transported to the surface of recombinant virus-infected Sf9 cells when examined by membrane-immunofluorescence analysis, and that the gD expressed on the surface of Sf9 cells adsorbed feline erythrocytes. Mice inoculated with a lysate of Sf9 cells expressing FHV-1 gD induced antibodies with virus-neutralizing and hemagglutination-inhibition activities. Therefore, the expressed gD appears to be biologically authentic. These data suggested that recombinant FHV-1 gD produced in Sf9 cells may be a useful immunogen as a feline vaccine. |
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Authors:
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K Maeda; M Ono; Y Kawaguchi; M Niikura; K Okazaki; N Yokoyama; Y Tokiyoshi; Y Tohya; T Mikami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virus research Volume: 46 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-04-14 Completed Date: 1997-04-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 75-80 Citation Subset: IM |
Affiliation:
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Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cat Diseases / virology Cats Erythrocytes / immunology, virology Hemagglutinins, Viral / biosynthesis, chemistry, genetics* Herpes Simplex / veterinary, virology Mice Neutralization Tests Nucleopolyhedrovirus / genetics* Recombinant Proteins / biosynthesis* Simplexvirus / genetics* Spodoptera / genetics Viral Envelope Proteins / biosynthesis, chemistry, genetics* |
| Chemical | |
Reg. No./Substance:
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0/Hemagglutinins, Viral; 0/Recombinant Proteins; 0/Viral Envelope Proteins; 0/glycoprotein D, Feline herpesvirus 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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