Document Detail


Expression and properties of feline herpesvirus type 1 gD (hemagglutinin) by a recombinant baculovirus.
MedLine Citation:
PMID:  9029779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We constructed a recombinant baculovirus expressing feline herpesvirus type I (FHV-1) gD in insect cells (Sf9 cells). The expressed product was identified as FHV-1 gD by a panel of monoclonal antibodies specific for the FHV-1 gD, and had an apparent molecular mass of approximately 49 kDa, which was less than that of the authentic FHV-1 gD. When the FHV-1 gD protein were expressed in Sf9 cells and CRFK cells in the presence of tunicamycin, the FHV-1 gD exhibited a molecular mass of 41 kDa. It was shown that the gD protein was transported to the surface of recombinant virus-infected Sf9 cells when examined by membrane-immunofluorescence analysis, and that the gD expressed on the surface of Sf9 cells adsorbed feline erythrocytes. Mice inoculated with a lysate of Sf9 cells expressing FHV-1 gD induced antibodies with virus-neutralizing and hemagglutination-inhibition activities. Therefore, the expressed gD appears to be biologically authentic. These data suggested that recombinant FHV-1 gD produced in Sf9 cells may be a useful immunogen as a feline vaccine.
Authors:
K Maeda; M Ono; Y Kawaguchi; M Niikura; K Okazaki; N Yokoyama; Y Tokiyoshi; Y Tohya; T Mikami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  46     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-04-14     Completed Date:  1997-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
Affiliation:
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cat Diseases / virology
Cats
Erythrocytes / immunology,  virology
Hemagglutinins, Viral / biosynthesis,  chemistry,  genetics*
Herpes Simplex / veterinary,  virology
Mice
Neutralization Tests
Nucleopolyhedrovirus / genetics*
Recombinant Proteins / biosynthesis*
Simplexvirus / genetics*
Spodoptera / genetics
Viral Envelope Proteins / biosynthesis,  chemistry,  genetics*
Chemical
Reg. No./Substance:
0/Hemagglutinins, Viral; 0/Recombinant Proteins; 0/Viral Envelope Proteins; 0/glycoprotein D, Feline herpesvirus 1

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