Document Detail

Expression and properties of feline herpesvirus type 1 gD (hemagglutinin) by a recombinant baculovirus.
MedLine Citation:
PMID:  9029779     Owner:  NLM     Status:  MEDLINE    
We constructed a recombinant baculovirus expressing feline herpesvirus type I (FHV-1) gD in insect cells (Sf9 cells). The expressed product was identified as FHV-1 gD by a panel of monoclonal antibodies specific for the FHV-1 gD, and had an apparent molecular mass of approximately 49 kDa, which was less than that of the authentic FHV-1 gD. When the FHV-1 gD protein were expressed in Sf9 cells and CRFK cells in the presence of tunicamycin, the FHV-1 gD exhibited a molecular mass of 41 kDa. It was shown that the gD protein was transported to the surface of recombinant virus-infected Sf9 cells when examined by membrane-immunofluorescence analysis, and that the gD expressed on the surface of Sf9 cells adsorbed feline erythrocytes. Mice inoculated with a lysate of Sf9 cells expressing FHV-1 gD induced antibodies with virus-neutralizing and hemagglutination-inhibition activities. Therefore, the expressed gD appears to be biologically authentic. These data suggested that recombinant FHV-1 gD produced in Sf9 cells may be a useful immunogen as a feline vaccine.
K Maeda; M Ono; Y Kawaguchi; M Niikura; K Okazaki; N Yokoyama; Y Tokiyoshi; Y Tohya; T Mikami
Related Documents :
10358729 - Replication of spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive ...
10233099 - Electrorotation studies of baby hamster kidney fibroblasts infected with herpes simplex...
3944589 - Semipermissive replication of adenovirus 5 in rat brain cells and evidence for an induc...
2997369 - The role of haemagglutinin-neuraminidase glycoprotein cell surface expression in the su...
810089 - Separation of viable rickettsia typhi from yolk sac and l cell host components by renog...
15099209 - Infection studies on human cell lines with porcine circovirus type 1 and porcine circov...
21273549 - Sonoporation enhances chemotherapeutic efficacy in retinoblastoma cells in vitro.
1506029 - A rapid method for flow cytometric cell counting and cell cycle analysis from monolayer...
3863439 - Fine needle aspiration of proliferative fasciitis. a case report.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  46     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-04-14     Completed Date:  1997-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cat Diseases / virology
Erythrocytes / immunology,  virology
Hemagglutinins, Viral / biosynthesis,  chemistry,  genetics*
Herpes Simplex / veterinary,  virology
Neutralization Tests
Nucleopolyhedrovirus / genetics*
Recombinant Proteins / biosynthesis*
Simplexvirus / genetics*
Spodoptera / genetics
Viral Envelope Proteins / biosynthesis,  chemistry,  genetics*
Reg. No./Substance:
0/Hemagglutinins, Viral; 0/Recombinant Proteins; 0/Viral Envelope Proteins; 0/glycoprotein D, Feline herpesvirus 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A dual role for endothelial cells in cytomegalovirus infection? A study of cytomegalovirus infection...
Next Document:  Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients.