Document Detail


Expression of proliferation-associated antigens (PCNA, p120, p145) during the reentry of G0 cells into the cell cycle.
MedLine Citation:
PMID:  8001459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flow cytometric bivariate analysis was used to evaluate the expression of PCNA, p120, and p145 during the G0 reentry of CHO-K1 cells into the cell cycle. CHO-K1 cells were placed in a G0-like state using serum depletion and stimulated to reenter the cell cycle by replating into fresh, serum-containing medium. At discrete intervals after stimulation, replicate samples were stained for either PCNA, p120, or 145; stained for DNA (Coulter DNA-Prep); evaluated on the EPICS Profile I; and analyzed on the EPICS ELITE workstation. PCNA stained less than 10% of the G0 cells; in contrast, however, 30-35% of the G0 cells were positive for p120 and p145. Eight hours after stimulating G0 cells to reenter the cell cycle (during G0/G1), p120 reached 88% positivity, while p145 and PCNA were 63% and 30% positive, respectively. Cells in S phase (12 and 16 h following G0 stimulation) were greater than 90% positive for all three antigens. PCNA had the greatest change throughout the G0 reentry process, both in percentage positive and quantitatively (mean channel fluorescence). This report indicates that all three proliferation-associated antigens studied are differentially expressed during the reentry of G0 cells into the cell cycle. Furthermore, these antigens may be useful in the early detection of G0 recruitment.
Authors:
W E Bolton; J W Freeman; W R Mikulka; C G Healy; R J Schmittling; N S Kenyon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  17     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-01-25     Completed Date:  1995-01-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  66-74     Citation Subset:  IM    
Affiliation:
Coulter Corporation, Miami, Florida 33116.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Biological Markers
CHO Cells / cytology
Cell Cycle
Cell Division
Cricetinae
Flow Cytometry*
Fluorescent Antibody Technique
Humans
Interphase*
Molecular Sequence Data
Nuclear Proteins / analysis*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
Proliferating Cell Nuclear Antigen / analysis*
S Phase*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Biological Markers; 0/NOL1 protein, human; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 0/proliferating cell nuclear antigen p145

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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