Document Detail


Expression profiling of metalloproteinases and their inhibitors in cartilage.
MedLine Citation:
PMID:  14730609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To profile the expression of all known members of the matrix metalloproteinase (MMP), ADAMTS, and tissue inhibitor of metalloproteinases (TIMP) gene families in normal cartilage and cartilage from patients with osteoarthritis (OA). METHODS: Human cartilage was obtained from femoral heads at joint replacement for OA or following fracture to the femoral neck. Total RNA was purified, and gene expression was assayed using quantitative real-time polymerase chain reaction. RESULTS: Several members of the above gene families were regulated in OA. Genes that showed increased expression in OA were MMP13, MMP28, and ADAMTS16 (all at P < 0.001), MMP9, MMP16, ADAMTS2, and ADAMTS14 (all at P < 0.01), and MMP2, TIMP3, and ADAMTS12 (all at P < 0.05). Genes with decreased expression in OA were MMP1, MMP3, and ADAMTS1 (all at P < 0.001), MMP10, TIMP1, and ADAMTS9 (all at P < 0.01), and TIMP4, ADAMTS5, and ADAMTS15 (all at P < 0.05). Correlation analysis revealed that groups of genes across the gene families were coexpressed in cartilage. CONCLUSION: This is the first comprehensive expression profile of all known MMP, ADAMTS, and TIMP genes in cartilage. Elucidation of patterns of expression provides a foundation with which to understand mechanisms of gene regulation in OA and potentially to refine the specificity of antiproteolytic therapies.
Authors:
Lara Kevorkian; David A Young; Clare Darrah; Simon T Donell; Lee Shepstone; Sarah Porter; Sarah M V Brockbank; Dylan R Edwards; Andrew E Parker; Ian M Clark
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  50     ISSN:  0004-3591     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-41     Citation Subset:  AIM; IM    
Affiliation:
University of East Anglia, Norwich, UK.
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MeSH Terms
Descriptor/Qualifier:
ADAM Proteins
Adult
Aged
Aged, 80 and over
Cartilage / physiology*
Disintegrins / genetics
Female
Femur / injuries
Fractures, Bone / genetics,  physiopathology
Gene Expression Profiling*
Humans
Male
Metalloendopeptidases / genetics
Metalloproteases / genetics*
Middle Aged
Osteoarthritis, Hip / genetics*,  physiopathology
Tissue Inhibitor of Metalloproteinases / genetics*
Chemical
Reg. No./Substance:
0/Disintegrins; 0/Tissue Inhibitor of Metalloproteinases; EC 3.4.-/Metalloproteases; EC 3.4.24.-/ADAM Proteins; EC 3.4.24.-/ADAMTS1 protein, human; EC 3.4.24.-/Metalloendopeptidases
Comments/Corrections
Comment In:
Arthritis Rheum. 2004 Dec;50(12):4074-5; author reply 4075   [PMID:  15593191 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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