Document Detail

Expression profiling of calcium induced genes in cultured human keratinocytes.
MedLine Citation:
PMID:  20358008     Owner:  NLM     Status:  MEDLINE    
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.
Jung-Suk Lee; Mi-Rang Kim; Nam-Soon Kim; Yong Sung Kim; Jun-Mo Yang; Ah Young Cho; Young Lee; Chang Deok Kim; Jeung-Hoon Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-19
Journal Detail:
Title:  Journal of Korean medical science     Volume:  25     ISSN:  1598-6357     ISO Abbreviation:  J. Korean Med. Sci.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-01     Completed Date:  2010-06-28     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8703518     Medline TA:  J Korean Med Sci     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  619-25     Citation Subset:  IM    
Department of Dermatology, School of Medicine, Chungnam National University, Jung-gu, Daejeon, Korea.
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MeSH Terms
Calcium / metabolism*
Cells, Cultured
Chromosome Mapping
Chromosomes, Human
Expressed Sequence Tags
Gene Expression Profiling
Gene Expression Regulation*
Gene Library
Keratinocytes / cytology,  physiology*
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Reg. No./Substance:

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