| Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet. | |
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MedLine Citation:
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PMID: 20412689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Göttingen minipig is an excellent model for studying effects of dietary high-fat intake on obesity. In this study, we analyzed the expression level of microRNA-122 (miRNA-122) and its target mRNA, CAT1, in intact young male minipigs fed either high-cholesterol or standard diet for 11 wk. MiRNA-122 and CAT1 are known to be important regulators of lipid metabolism. The weight of the young minipigs was monitored once a week during the feeding period; measurements of total cholesterol, triglycerides, high-density lipoproteins, and low-density lipoproteins were recorded at 4 time points (8, 14, 16, and 19 wk of age) in fasting animals during the feeding scheme. Body weight, total cholesterol, and high-density lipoproteins were higher in pigs fed the high-cholesterol compared with the standard diet. In contrast, the level of triglycerides was lower in pigs on the high-cholesterol diet than those receiving the standard diet. Pigs fed high-cholesterol also had lower miRNA-122 levels than did those fed the standard diet. These results suggest that in our minipigs, the increase in weight and cholesterol levels resulting from subchronic (11 wk) feeding of a high-cholesterol diet is correlated with a decrease in the expression of miRNA-122, confirming the implication of this microRNA in obesity. Gene expression levels of CAT1 did not differ between groups. |
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Authors:
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Susanna Cirera; Malene Birck; Peter K Busk; Merete Fredholm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Comparative medicine Volume: 60 ISSN: 1532-0820 ISO Abbreviation: Comp. Med. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-23 Completed Date: 2010-07-30 Revised Date: 2011-06-20 |
Medline Journal Info:
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Nlm Unique ID: 100900466 Medline TA: Comp Med Country: United States |
Other Details:
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Languages: eng Pagination: 136-41 Citation Subset: IM |
Affiliation:
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Department of Basic Animal and Veterinary Sciences, Division of Genetics and Bioinformatics, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark. scs@life.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Body Weight Cationic Amino Acid Transporter 1 / genetics, metabolism* Cholesterol / blood Cholesterol, Dietary* Diet Fasting Female Lipoproteins, LDL / blood Male MicroRNAs / genetics, metabolism* Random Allocation Sus scrofa Swine Swine, Miniature* / genetics, metabolism Triglycerides / blood |
| Chemical | |
Reg. No./Substance:
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0/Cationic Amino Acid Transporter 1; 0/Cholesterol, Dietary; 0/Lipoproteins, LDL; 0/MicroRNAs; 0/Triglycerides; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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