| Expression profiles of the glucose-dependent insulinotropic peptide receptor and LHCGR in sporadic adrenocortical tumors. | |
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MedLine Citation:
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PMID: 18971217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucose-dependent insulinotropic peptide receptor (GIPR) and LHCGR are G-protein-coupled receptors with a wide tissue expression pattern. Aberrant expression of these receptors has rarely been demonstrated in adult sporadic adrenocortical tumors with a lack of data on pediatric tumors. We quantified the GIPR and LHCGR expression in a large cohort of 55 patients (25 children and 30 adults) with functioning and non-functioning sporadic adrenocortical tumors. Thirty-eight tumors were classified as adenomas whereas 17 were carcinomas. GIPR and LHCGR expression were analyzed by real-time PCR and normal human pancreatic and testicular tissue samples were used as positive controls. Mean expression values were determined by fold increase in comparison with a normal adrenal pool. GIPR mRNA levels were significantly higher in adrenocortical carcinomas than in adenomas from both pediatric and adult groups. LHCGR expression was similar in both carcinomas and adenomas from the pediatric group but significantly lower in carcinomas than in adenomas from the adult group (median 0.06 and 2.3 respectively, P<0.001). GIPR was detected by immunohistochemistry in both pediatric and adult tumors. Staining and real-time PCR results correlated positively only when GIPR mRNA levels were increased at least two-fold in comparison with normal adrenal expression levels. In conclusion, GIPR overexpression was observed in pediatric and adult adrenocortical tumors and very low levels of LHCGR expression were found in all adult adrenocortical carcinomas. |
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Authors:
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Marcia Helena Soares Costa; Ana Claudia Latronico; Regina Matsunaga Martin; Angela S Barbosa; Madson Q Almeida; Claudimara Ferini Pacicco Lotfi; Helena P Lima Valassi; Mirian Yumie Nishi; Antonio Marmo Lucon; Sheila Aparecida Siqueira; Maria Claudia Nogueira Zerbini; Luciani Renata Carvalho; Berenice Bilharinho Mendonca; Maria Candida Barisson Villares Fragoso |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-29 |
Journal Detail:
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Title: The Journal of endocrinology Volume: 200 ISSN: 1479-6805 ISO Abbreviation: J. Endocrinol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-03 Completed Date: 2009-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 167-75 Citation Subset: IM |
Affiliation:
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Laboratório de Hormônios e Genética Molecular LIM/42, Divisão de Endocrinologia e Metabologia, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, Avenue Dr Eneas de Carvalho Aguiar, São Paulo, SP, Brazil. mhsc@usp.br |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adrenal Cortex Neoplasms / genetics* Adrenocortical Adenoma / genetics* Adrenocortical Carcinoma / genetics* Adult Aged Child Child, Preschool Female Gene Expression Gene Expression Regulation, Neoplastic / genetics* Humans Infant Male Middle Aged Receptors, Gastrointestinal Hormone / genetics* Receptors, LH / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Gastrointestinal Hormone; 0/Receptors, LH; 0/gastric inhibitory polypeptide receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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