Document Detail


Expression profiles of Na+,K+-ATPase during acute and chronic hypo-osmotic stress in the blue crab Callinectes sapidus.
MedLine Citation:
PMID:  16946242     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During acclimation to dilute seawater, the specific activity of Na+,K+-ATPase increases substantially in the posterior gills of the blue crab Callinectes sapidus. To determine whether this increase occurs through regulation of pre-existing enzyme or synthesis of new enzyme, mRNA and protein levels were measured over short (<24 h) and long (18 days) time courses. Na+,K+-ATPase expression, both mRNA and protein, did not change during the initial 24-h exposure to dilute seawater (10 ppt salinity). Thus, osmoregulation in C. sapidus during acute exposure to low salinity likely involves either modulation of existing enzyme or mechanisms other than an increase in the amount of Na+,K+-ATPase enzyme. However, crabs exposed to dilute seawater over 18 days showed a 300% increase in Na+,K+-ATPase specific activity as well as a 200% increase in Na+,K+-ATPase protein levels. Thus, it appears that the increase in Na+,K+-ATPase activity during chronic exposure results from the synthesis of new enzyme. The relative amounts of mRNA for the alpha-subunit increased substantially (by 150%) during the acclimation process, but once the crabs had fully acclimated to low salinity, the mRNA levels had decreased and were not different from levels in crabs fully acclimated to high salinity. Thus, there is transient induction of the Na+,K+-ATPase mRNA levels during acclimation to dilute seawater.
Authors:
Donald L Lovett; Michael P Verzi; Joseph E Burgents; Christopher A Tanner; Krzysztof Glomski; Joan J Lee; David W Towle
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Biological bulletin     Volume:  211     ISSN:  0006-3185     ISO Abbreviation:  Biol. Bull.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-01     Completed Date:  2007-01-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984727R     Medline TA:  Biol Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-65     Citation Subset:  IM    
Affiliation:
Department of Biology, The College of New Jersey, Ewing, New Jersey 08628, USA. lovett@tcnj.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Brachyura / enzymology*
Gene Expression Profiling*
Gene Expression Regulation, Enzymologic*
Osmolar Concentration
RNA, Messenger
Sodium-Potassium-Exchanging ATPase / metabolism*
Stress, Physiological / enzymology*
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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