Document Detail


Expression and possible role of 20alpha-hydroxysteroid dehydrogenase in the placenta of the goat.
MedLine Citation:
PMID:  15703459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
20Alpha-hydroxysteroid dehydrogenase (20alpha-HSD) catalyzes the conversion of progesterone to its inactive form 20alpha-dihydroprogesterone (20alpha-OHP). 20Alpha-HSD is expressed in the murine placenta, suggesting a role, yet unidentified, played by this enzyme during the course of pregnancy. To elucidate the possible roles of 20alpha-HSD during pregnancy, 20alpha-HSD gene expression in the placenta was examined by Northern blot analysis, and progestin (progesterone and 20alpha-OHP) concentrations in the maternal and fetal sera and the amniotic fluid were measured by radioimmunoassay in pregnant Shiba goats. The expression of 20alpha-HSD mRNA was observed in the placenta and the intercaruncular part of the uterus during mid to late pregnancy. Analysis by in situ hybridization revealed that 20alpha-HSD mRNA was mainly localized in the endometrial epithelium of the caruncle side of the placenta. Considerable enzyme activity of 20alpha-HSD was also detected in the cytosolic fraction of the placenta and intercaruncular part of the uterus. Although concentrations of progesterone and 20alpha-OHP in the maternal serum showed similar profiles, progesterone levels in the fetal serum stayed extremely low throughout the pregnancy. The 20alpha-OHP concentration in the fetal serum was always higher than that in the maternal serum. In the amniotic fluid, the concentrations of both progesterone and 20alpha-OHP remained at very low levels throughout the pregnancy. These results support the notion that 20alpha-HSD protects the fetus from the cytotoxic effects of progesterone, and thereby maintains the normal development of the fetus.
Authors:
Walimuni Samantha Nilanthi Jayasekara; Tomohiro Yonezawa; Maho Ishida; Keitaro Yamanouchi; Masugi Nishihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-02-10
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  51     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-10     Completed Date:  2005-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  265-72     Citation Subset:  IM    
Affiliation:
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
20-alpha-Hydroxysteroid Dehydrogenase / genetics*,  metabolism*
Amniotic Fluid / metabolism
Animals
Female
Fetal Blood / metabolism
Gene Expression Regulation, Enzymologic
Goats / physiology*
Placenta / enzymology*
Pregnancy
Progesterone / blood
RNA, Messenger / analysis
Uterus / enzymology
Chemical
Reg. No./Substance:
0/RNA, Messenger; 57-83-0/Progesterone; EC 1.1.1.149/20-alpha-Hydroxysteroid Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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