Document Detail

Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines.
MedLine Citation:
PMID:  23197999     Owner:  NLM     Status:  Publisher    
Previous studies have demonstrated that pluripotency-associated transcription factors, such as Oct3/4, Nanog and Sox2, play a crucial role in the development and malignant progression of various types of tumors. Breast cancer is the most frequent cancer among females, being a heterogeneous disease, with distinct morphologies, metastatic behavior and therapeutic responses. The expression of Oct3/4, Nanog and Sox2 in 3 human breast cancer cell lines, MCF7, T-47D and MDA-MB-231, was determined. The expression of Oct3/4, Nanog and Sox2 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR) and protein expression was detected by immunocytohistochemistry. RT-PCR revealed that all three human breast cancer cell lines tested expressed evident Oct3/4, Nanog and Sox-2 mRNA at various levels. Higher levels of Oct3/4 were identified in MCF7 and MDA-MB-231 cells compared with T-47D cells. Higher levels of Nanog were observed in MCF7 and T-47D cells compared with MDA-MB-231 cells and the highest expression of Sox-2 was detected in MCF7 cells. The nuclear localization of Oct3/4, Nanog and Sox-2 was confirmed by immunostaining. Oct3/4, Nanog and Sox2 were expressed in human breast cancer cell lines. Further studies are required to characterize the role of Oct3/4, Nanog and Sox2 in human breast cancer.
Gui-Qin Ling; Dong-Bo Chen; Bao-Qing Wang; Lan-Sheng Zhang
Related Documents :
24127549 - Regulation of histone h2a.z expression is mediated by sirtuin 1 in prostate cancer.
22606439 - Local recurrence of breast cancer 52 years after halsted mastectomy: is there a role fo...
23921149 - 3,5,4'-trimethoxystilbene, a natural methoxylated analogue of resveratrol, inhibits bre...
22674239 - Relevance and completeness of the international classification of functioning, disabili...
24518199 - Artesunate induces g2/m cell cycle arrest through autophagy induction in breast cancer ...
23919629 - Clinical, cytologic, and histologic features of a mammary micropapillary carcinoma in a...
14573709 - Breast cancer risk in airline cabin attendants: a nested case-control study in iceland.
9406579 - A novel brca1 mutation in an identical twin pair with similar clinical histories.
16740699 - Developmental exposure to estradiol and bisphenol a increases susceptibility to prostat...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-13
Journal Detail:
Title:  Oncology letters     Volume:  4     ISSN:  1792-1082     ISO Abbreviation:  Oncol Lett     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101531236     Medline TA:  Oncol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1264-1268     Citation Subset:  -    
The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R.China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Perforated gastrointestinal stromal tumor in the jejunum: A rare cause of acute abdomen.
Next Document:  Cryptosporidium in countries of the Arab world: the past decade (2002-2011).