| Expression of platelet-derived growth factor and its receptors in neuroendocrine tumors of the digestive system. | |
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MedLine Citation:
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PMID: 1310635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carcinoid tumors are slowly growing neuroendocrine neoplasms which often present pronounced fibrosis around the tumor cells. We have previously shown by immunohistochemistry that carcinoid tumors express platelet-derived growth factor (PDGF) beta-receptors on surrounding stromal cells. In this report, 22 midgut carcinoids and 5 endocrine pancreatic tumors were examined for the presence of PDGF with a monoclonal antibody raised against a peptide corresponding to a part of the B-chain of PDGF which reacts strongly with the B-chain and weakly with the A-chain. They were also examined for PDGF alpha-receptors with an affinity-purified polyclonal peptide antibody and for PDGF beta-receptor with the monoclonal antibody PDGFR-B2. PDGF was expressed on tumor cells and on adjacent stroma. PDGF alpha-receptor was seen on clusters of tumor cells and occasionally on adjacent stroma, whereas beta-receptors were seen only in the stroma. Tissue sections from some of these midgut carcinoids were also investigated by in situ hybridization for mRNA of PDGF A- and B-chains as well as alpha- and beta-receptors. By in situ hybridization, abundant expression of mRNA for PDGF beta-receptor and PDGF A-chain was observed in stromal cells adjacent to carcinoid tumor cell clusters, but the mRNA expression in the tumor cells themselves was at a low level. A few clustered tumor cells and stromal cells expressed mRNA for the PDGF alpha-receptor, thus consolidating the immunohistochemical findings. mRNA for the PDGF B-chain was detected in both tumor cells and stroma, but only at low levels. Our data suggest that PDGF is involved in the growth stimulation of the carcinoid tumor cells in an autocrine fashion and in the stimulation of stromal cell growth through paracrine and possibly autocrine mechanisms. Moreover, remarkably strong immunostaining of PDGF and the PDGF alpha-receptor was seen on peripheral nerve fibers. |
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Authors:
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A Chaudhry; V Papanicolaou; K Oberg; C H Heldin; K Funa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 52 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-03-17 Completed Date: 1992-03-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1006-12 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University Hospital, Uppsala, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antibodies, Monoclonal Carcinoid Tumor / metabolism, pathology* Humans Immunoenzyme Techniques Immunohistochemistry Intestinal Neoplasms / metabolism, pathology* Macromolecular Substances Middle Aged Nucleic Acid Hybridization Pancreatic Neoplasms / metabolism, pathology* Platelet-Derived Growth Factor / analysis, genetics, metabolism* RNA Probes RNA, Messenger / analysis, genetics Receptors, Cell Surface / analysis, genetics, metabolism* Receptors, Platelet-Derived Growth Factor |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Macromolecular Substances; 0/Platelet-Derived Growth Factor; 0/RNA Probes; 0/RNA, Messenger; 0/Receptors, Cell Surface; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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