Document Detail


Expression of phaseolin cDNA genes in yeast under control of natural plant DNA sequences.
MedLine Citation:
PMID:  16593534     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
We have constructed a strain of Saccharomyces cerevisiae that expresses two different members of the multigene family encoding phaseolin, the major seed storage glycoprotein from the French bean, Phaseolus vulgaris. Yeast vector plasmids have been engineered to include a Phaseolus DNA segment that contains the natural 5' and 3' plant genomic regulatory sequences flanking a cDNA copy of the protein-encoding region. Characterization of phaseolin transcripts isolated from transformed yeast cells revealed the presence of two classes of polyadenylylated RNA, approximately 1400 and 1800 bases, which initiate and terminate in plant DNA sequences. Protein extracts from transformants contain phaseolin-immunoreactive proteins similar in size to those isolated from plant tissue. These polypeptides are glycosylated in yeast and their molecular weights are consistent with the possibility that the phaseolin signal peptide has been cleaved to form the mature protein.
Authors:
J H Cramer; K Lea; J L Slightom
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-06-30     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  334-8     Citation Subset:  -    
Affiliation:
Agrigenetics Advanced Research Division, 5649 East Buckeye Road, Madison, WI 53716.
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