Document Detail


Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations.
MedLine Citation:
PMID:  1356428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To verify the physiological role of the pore-forming protein perforin in vivo, its expression in subpopulations of human peripheral blood lymphocytes was examined by immunocytochemical staining and their cytolytic potentials compared. In addition to NK cells and gamma delta T cells, which uniformly expressed abundant perforin in their cytoplasmic granules, only a small subpopulation of CD8+ alpha beta T cells contained perforin, namely the CD11b+ subset. However, in vitro activation with an anti-CD3 antibody and IL-2 induced perforin expression in approximately 50% of the CD8+CD11b- T cells and also in a small subset of CD4+ T cells. A distribution of perforin in CD8+ and CD4+ T cells, similar to in vitro activated T cells, was observed in fresh peripheral blood lymphocytes from infectious mononucleosis patients. In all instances, the expression of perforin correlated with the cytolytic potential of these subpopulations. The results strongly suggest that perforin plays a role in the manifestation of cytotoxic activity in vivo.
Authors:
M Nakata; A Kawasaki; M Azuma; K Tsuji; H Matsuda; Y Shinkai; H Yagita; K Okumura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International immunology     Volume:  4     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-11-16     Completed Date:  1992-11-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1049-54     Citation Subset:  IM    
Affiliation:
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Antigens, CD11
Antigens, CD8 / analysis
Cytotoxicity, Immunologic*
Humans
Infectious Mononucleosis / immunology
Lymphocyte Activation
Lymphocyte Subsets / chemistry,  immunology*
Membrane Glycoproteins*
Membrane Proteins / analysis*
Perforin
Pore Forming Cytotoxic Proteins
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD11; 0/Antigens, CD8; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Pore Forming Cytotoxic Proteins; 126465-35-8/Perforin

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