Document Detail


Expression of pax6 and sox2 in adult olfactory epithelium.
MedLine Citation:
PMID:  20852734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The olfactory epithelium maintains stem and progenitor cells that support the neuroepithelium's life-long capacity to reconstitute after injury. However, the identity of the stem cells--and their regulation--remain poorly defined. The transcription factors Pax6 and Sox2 are characteristic of stem cells in many tissues, including the brain. Therefore, we assessed the expression of Pax6 and Sox2 in normal olfactory epithelium and during epithelial regeneration after methyl bromide lesion or olfactory bulbectomy. Sox2 is found in multiple kinds of cells in normal epithelium, including sustentacular cells, horizontal basal cells, and some globose basal cells. Pax6 is co-expressed with Sox2 in all these, but is also found in duct/gland cells as well as olfactory neurons that innervate necklace glomeruli. Most of the Sox2/Pax6-positive globose basal cells are actively cycling, but some express the cyclin-dependent kinase inhibitor p27(Kip1), and are presumably mitotically quiescent. Among globose basal cells, Sox2 and Pax6 are co-expressed by putatively multipotent progenitors (labeled by neither anti-Mash1 nor anti-Neurog1) and neuron-committed transit amplifying cells (which express Mash1). However, Sox2 and Pax6 are expressed by only a minority of immediate neuronal precursors (Neurog1- and NeuroD1-expressing). The assignment of Sox2 and Pax6 to these categories of globose basal cells is confirmed by a temporal analysis of transcription factor expression during the recovery of the epithelium from methyl bromide-induced injury. Each of the Sox2/Pax6-colabeled cell types is at a remove from the birth of neurons; thus, suppressing their differentiation may be among the roles of Sox2/Pax6 in the olfactory epithelium.
Authors:
Zhen Guo; Adam Packard; Richard C Krolewski; Margaret T Harris; Glen L Manglapus; James E Schwob
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  518     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-01-13     Revised Date:  2013-11-15    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4395-418     Citation Subset:  IM    
Affiliation:
Cell, Molecular, and Developmental Biology Graduate Program, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA 02111, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors / metabolism
Eye Proteins / genetics,  metabolism*
Homeodomain Proteins / genetics,  metabolism*
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nerve Regeneration
Nerve Tissue Proteins / metabolism
Olfactory Mucosa / cytology,  metabolism*
Paired Box Transcription Factors / genetics,  metabolism*
Rats
Rats, Sprague-Dawley
Repressor Proteins / genetics,  metabolism*
SOXB1 Transcription Factors / genetics,  metabolism*
Sensory Receptor Cells / cytology,  metabolism
Stem Cells / cytology,  physiology
Grant Support
ID/Acronym/Agency:
DC002167/DC/NIDCD NIH HHS; F30 DC010276/DC/NIDCD NIH HHS; F30 DC010276-01/DC/NIDCD NIH HHS; F30 DC010276-02/DC/NIDCD NIH HHS; F30 DC010276-03/DC/NIDCD NIH HHS; R01 DC002167/DC/NIDCD NIH HHS; R01 DC002167-10/DC/NIDCD NIH HHS; R01 DC002167-11/DC/NIDCD NIH HHS; R01 DC002167-12/DC/NIDCD NIH HHS; R01 DC002167-13/DC/NIDCD NIH HHS; R01 DC002167-14/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Ascl1 protein, mouse; 0/Ascl1 protein, rat; 0/Basic Helix-Loop-Helix Transcription Factors; 0/Eye Proteins; 0/Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Repressor Proteins; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse; 0/Sox2 protein, rat; 182238-50-2/Neurog1 protein, mouse
Comments/Corrections

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