Document Detail


Expression patterns of the ectopeptidases aminopeptidase N/CD13 and dipeptidyl peptidase IV/CD26: immunoultrastructural topographic localization on different types of cultured cells.
MedLine Citation:
PMID:  9587627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aminopeptidase N/CD13 and dipeptidyl peptidase IV/CD26 are widespread membrane-bound peptidases involved in fundamental biological processes. Using cryo-ultramicrotomy of cultured cells followed by indirect immunogold labelling, both enzymes appeared to be strongly and regularly labelled on the cell surfaces of human synovial fibroblasts, T-lymphocytes and colon carcinoma cells Caco-2. In the cytoplasm of the synovial fibroblasts gold-labelled vesicle-like structures were found, which we consider to be potential transport vesicles. An abundant and regular expression of CD13 was detected on cultured renal parenchymal cells. On the renal carcinoma cell line Caki-1 cells we found a low, non-homogeneous and clustered CD13-labelling. On cultured renal parenchymal cells and the Caki-1 cells CD26 could not be observed. The expression pattern of CD26 on renal carcinoma cell line Caki-2 cells showed also a slightly clustered distribution. A low density CD26-labelling was present on the squamous cell carcinoma cell line UM-SCC-22B. CD13 was absent in Caki-2 and UM-SCC-22B cells. The presence of both enzymes on the cultured cells enables their ultrastructural investigation under different growth conditions and their involvement in cell-cell interactions. For this purpose, however, further investigations are necessary.
Authors:
T Stange; U Kettmann; H J Holzhausen; D Riemann
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta histochemica     Volume:  100     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-07-14     Completed Date:  1998-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  157-69     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Medical School, University of Halle, Germany.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD13 / biosynthesis*
Antigens, CD26 / biosynthesis*
Caco-2 Cells
Carcinoma / chemistry,  enzymology,  ultrastructure
Cells, Cultured
Fibroblasts
Humans
Immunohistochemistry
Kidney Neoplasms / chemistry,  enzymology,  ultrastructure
Microscopy, Immunoelectron
Synovial Fluid / cytology,  enzymology
T-Lymphocytes
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
EC 3.4.11.2/Antigens, CD13; EC 3.4.14.5/Antigens, CD26

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