Document Detail

Expression pattern and sub-cellular distribution of phosphoinositide specific phospholipase C enzymes after treatment with U-73122 in rat astrocytoma cells.
MedLine Citation:
PMID:  20564200     Owner:  NLM     Status:  MEDLINE    
Phosphoinositide specific phospholipase C (PI-PLC) enzymes interfere with the metabolism of inositol phospholipids (PI), molecules involved in signal transduction, a complex process depending on various components. Many evidences support the hypothesis that, in the glia, isoforms of PI-PLC family display different expression and/or sub cellular distribution under non-physiological conditions such as the rat astrocytes activation during neurodegeneration, the tumoural progression of some neoplasms and the inflammatory cascade activation after lipopolysaccharide administration, even if their role remains not completely elucidated. Treatment of a cultured established glioma cell line (C6 rat astrocytoma cell line) induces a modification in the pattern of expression and of sub cellular distribution of PI-PLCs compared to untreated cells. Special attention require PI-PLC beta3 and PI-PLC gamma2 isoforms, whose expression and sub cellular localization significantly differ after U-73122 treatment. The meaning of these modifications is unclear, also because the use of this N-aminosteroid compound remains controversial, inasmuch it has further actions which might contribute to the global effect recorded on the treated cells.
Vincenza Rita Lo Vasco; Cinzia Fabrizi; Barbara Panetta; Lorenzo Fumagalli; Lucio Cocco
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  110     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1005-12     Citation Subset:  IM    
Copyright Information:
J. Cell. Biochem. 110: 1005-1012, 2010. (c) 2010 Wiley-Liss, Inc.
Department of Otorinolaringoiatria, Audiologia and Foniatria "G. Ferreri", Policlinico Umberto I, Rome, Italy.
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MeSH Terms
Astrocytoma / enzymology,  pathology*
Base Sequence
Cell Line, Tumor
DNA Primers
Estrenes / pharmacology*
Gene Expression Regulation / drug effects*
Microscopy, Fluorescence
Phosphoinositide Phospholipase C / metabolism*
Pyrrolidinones / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Subcellular Fractions / drug effects*,  enzymology
Reg. No./Substance:
0/DNA Primers; 0/Estrenes; 0/Pyrrolidinones; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; EC Phospholipase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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