Document Detail


Expression pattern of sonic hedgehog and effect of topical mitomycin C on its expression in human ocular surface neoplasms.
MedLine Citation:
PMID:  18661269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To examine if the cells of human ocular surface neoplasms express sonic hedgehog (Shh) and the effects of topical mitomycin C on its expression. METHODS: Conjunctival tissues obtained from two normal subjects, two patients with squamous cell carcinoma of the ocular surface (conjunctiva), and one patient with ocular epithelial dysplasia were used in this study. Histological sections were processed for light microscopic immunohistochemical analysis for Shh. RESULTS: Faint immunoreactivity for Shh was detected in basal epithelial cells of limbus, bulbar, and palpebral conjunctival epithelial cells. On the other hand, squamous cell carcinoma cells markedly expressed Shh with positive staining for Patched 1(Ptc), the cell surface receptor of Shh. Similar marked expression of Shh was detected in the patient with ocular epithelial dysplasia, and this Shh expression was almost eliminated following topical mitomycin C treatment. A cell culture experiment was conducted to examine the effect of mitomycin C on Shh expression in a cultured squamous cell carcinoma cell line. CONCLUSIONS: Conjunctival epithelium constitutively expresses a low level of Shh, and its expression increases during malignant conversion of epithelial cells. Reduction of Shh expression might be involved in the therapeutic efficacy of topical mitomycin C for ocular surface epithelial neoplasms.
Authors:
Kyoko Fujita; Takeshi Miyamoto; Yuka Okada; Nobuyuki Ishikawa; Shizuya Saika
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Publication Detail:
Type:  Journal Article     Date:  2008-07-27
Journal Detail:
Title:  Japanese journal of ophthalmology     Volume:  52     ISSN:  0021-5155     ISO Abbreviation:  Jpn. J. Ophthalmol.     Publication Date:    2008 May-Jun
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0044652     Medline TA:  Jpn J Ophthalmol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  190-4     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan. kyoko@wakayama-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Aged
Antibiotics, Antineoplastic / therapeutic use*
Carcinoma, Squamous Cell / drug therapy*,  metabolism,  pathology
Conjunctival Neoplasms / drug therapy*,  metabolism,  pathology
Corneal Diseases / drug therapy*,  metabolism,  pathology
Epithelium, Corneal / drug effects,  metabolism,  pathology
Female
Fluorescent Antibody Technique, Indirect
Hedgehog Proteins / metabolism*
Humans
Male
Middle Aged
Mitomycin / therapeutic use*
Tumor Cells, Cultured
Up-Regulation
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Hedgehog Proteins; 0/SHH protein, human; 50-07-7/Mitomycin

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