Document Detail

Expression pattern of recombinant organophosphorus hydrolase from Flavobacterium sp. ATCC 27551 in Escherichia coli.
MedLine Citation:
PMID:  23274957     Owner:  NLM     Status:  Publisher    
Concerned with the influence of tagging system on the expression of heterogeneous protein in Escherichia coli, we attempted to express the organophosphorus hydrolase (OPH) of Flavobacterium sp. ATCC 27551 in E. coli. Recombinant OPH was overproduced successfully in E. coli when modified without the use of a tobacco etch virus (TEV) protease cleavage sequence. In addition, though there has never been a report on the extracellular secretion of recombinant OPH harboring native Tat signal peptides in E. coli, the produced protein was observed to be secreted extracellularly. Through the use of reverse transcriptional quantitative real-time PCR and comparison of the predicted folding rate, it was determined that OPH expression may be affected by the existence of a TEV protease cleavage sequence at the C-terminus during the process of translated protein folding, leading to the suppressed OPH activity. With the potential compatibility between native Tat signal peptides of OPH and E. coli Tat pathway secretion system, we report a successful expression of recombinant OPH harboring native Tat signal peptides in E. coli, for the first time.
Yunyoung Kwak; In-Koo Rhee; Jae-Ho Shin
Related Documents :
8147897 - The binding of fibronectin to entactin is mediated through the 29 kda amino terminal fr...
3059347 - High-level expression of a bioengineered, cysteine-free hepatocyte-stimulating factor (...
14736427 - Production of a biotinylated single-chain antibody fragment in the cytoplasm of escheri...
16155117 - N-glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for pr...
6734597 - Characterization of a novel killer toxin encoded by a double-stranded linear dna plasmi...
106887 - Purification and preparation of antibody to rna polymerase ii stimulatory factors from ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-29
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Extending prednisolone treatment does not reduce relapses in childhood nephrotic syndrome.
Next Document:  Biodegradation of the neonicotinoid insecticide thiamethoxam by the nitrogen-fixing and plant-growth...