Document Detail

Expression of p63 in conjunctival intraepithelial neoplasia and squamous cell carcinoma.
MedLine Citation:
PMID:  16021499     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: p63 is a homologue of the tumour suppressor gene p53, which is expressed in human basal squamous epithelium. Some investigators maintain that p63 plays a role in the development of squamous epithelium and, despite its homology to p53, it is considered to act as an oncogene. This study investigated the expression of p63 in conjunctival intraepithelial neoplasia of different grades, and conjunctival squamous cell carcinoma and its correlation to the proliferation marker MIB-1. MATERIAL AND METHODS: Seventeen conjunctival specimens excised with the suspicion of either conjunctival intraepithelial neoplasia (CIN) or squamous cell carcinoma were diagnosed histologically as follows: 2 squamous cell carcinomas of the conjunctiva, 2 CIN grade I, 3 CIN grade II, 7 CIN grade III, 2 CIN with beginning invasion and 1 normal conjunctiva with no dysplasia. Sixteen microscopically-normal postmortem conjunctival specimens and normal conjunctiva, CIN and carcinoma specimens were stained immunohistochemically with antibodies against p63 and MIB-1. At least 500 cells per specimen were counted and the percentage of positively-stained cells of each antibody was calculated. RESULTS: A mean of 80% (57-89%) of the dysplastic cells from the CIN specimens stained positively with antibodies against p63, especially in the lower two-thirds of the epithelium, statistically significantly more compared with the normal specimens (9-55%, mean 36%, p<0.001). Nevertheless, we did not find a correlation between the percentage of p63-positive cells and the differentiation grade of the malignant specimens. MIB-1 positivity was shown by 0-1% of the cells in the normal postmortem controls, by 3-30% (mean 12%) of the cells in the basal and occasionally in the middle layer of the CIN specimens, and 16-61% (mean 23%) in the carcinoma specimens. CONCLUSION: In conjunctival intraepithelial neoplasia and squamous cell carcinoma of the conjunctiva, p63 is preferentially expressed in the immature dysplastic epithelial cells. Its staining does not correlate with MIB-1-expression, and therefore does not appear to be linked to cell proliferation.
Claudia Auw-Haedrich; Rainer Sundmacher; Nikolaus Freudenberg; Helga Spelsberg; Nicolas Feltgen; Philip Maier; Thomas Reinhard
Related Documents :
8306659 - Suppression of human corneal epithelial proliferation with breast carcinoma immunotoxin.
15448749 - Chromosomal aberrations accumulate in polyploid cells of high-grade squamous intraepith...
1315439 - Comparison of southern blot analysis with isotopic and nonisotopic in situ hybridizatio...
860099 - Cytotoxic antibody in the serum of cattle with squamous cell carcinoma.
18194839 - P63 contributes to cell invasion and migration in squamous cell carcinoma of the head a...
14617039 - A novel b*38 allele, b*3809, was identified via sequence -based typing of b-cell line n...
9002999 - The interaction between beta-endorphin and gonadal steroids in regulation of luteinizin...
1566859 - Expression and transport of rabbit surfactant protein a in cos-1 cells.
52399 - Role of cell wall topography in conjugation of schizosaccharomyces pombe.
Publication Detail:
Type:  Journal Article     Date:  2005-07-15
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  244     ISSN:  0721-832X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-11-30     Completed Date:  2006-03-14     Revised Date:  2008-06-27    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  96-103     Citation Subset:  IM    
Eye Hospital, Albert-Ludwigs-University, 79106 Freiburg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aged, 80 and over
Carcinoma in Situ / metabolism*,  pathology
Carcinoma, Squamous Cell / metabolism*,  pathology
Conjunctival Neoplasms / metabolism*,  pathology
DNA-Binding Proteins
Genes, Tumor Suppressor
Immunoenzyme Techniques
Ki-67 Antigen / metabolism
Middle Aged
Phosphoproteins / metabolism*
Trans-Activators / metabolism*
Tumor Suppressor Proteins
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Ki-67 Antigen; 0/Phosphoproteins; 0/TP63 protein, human; 0/Trans-Activators; 0/Tumor Suppressor Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Two patients with different features of congenital optic disc anomalies in the two eyes.
Next Document:  Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice (Oryza sati...