| Expression of p53 and flow cytometric DNA analysis of isolated neoplastic glands of the stomach: an application of the gland isolation method. | |
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MedLine Citation:
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PMID: 7655735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression of p53 was studied immunohistochemically in combination with the DNA ploidy pattern by gland isolation in 97 alcohol-fixed gastric lesions. A polyclonal antibody, CM-1, was applied to the paraffin-embedded sections in this study. Overexpression of the p53 protein was found in 73.2% of 41 well or moderately differentiated gastric carcinomas and 52.2% of 23 cases with poor differentiation (P < 0.05). Immunoreactivity of p53 was also detected in isolated cancerous glands. No p53 immunoreactivity was detected in benign gastric lesions including adenomas, hyperplastic polyps and regions of intestinal metaplasia. In addition, flow cytometric DNA analysis was performed on isolated glandular epithelium adjacent to the portions used for immunostaining. DNA aneuploidy (DA) was detected in 85.7% of the well or moderately differentiated carcinomas and 42.9% of those with poor differentiation (P < 0.05). There was a positive correlation between DA, p53 positivity and the presence of regional lymph node metastasis, but not with other clinicopathological variables. In spite of the limited applicability of this method to poorly differentiated gastric cancer, we found that immunostaining and flow cytometry in combination with the gland isolation method facilitates analysis of gastric carcinogenesis. |
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Authors:
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Y Kitayama; H Sugimura; M Tanaka; S Nakamura; I Kino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virchows Archiv : an international journal of pathology Volume: 426 ISSN: 0945-6317 ISO Abbreviation: Virchows Arch. Publication Date: 1995 |
Date Detail:
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Created Date: 1995-10-02 Completed Date: 1995-10-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423843 Medline TA: Virchows Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 557-62 Citation Subset: IM |
Affiliation:
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First Department of Pathology, Hamamatsu University School of Medicine, Shizouka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Neoplasm
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genetics* Female Flow Cytometry Humans Male Middle Aged Ploidies Stomach Neoplasms / chemistry*, genetics* Tumor Suppressor Protein p53 / analysis* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Tumor Suppressor Protein p53 |
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