Document Detail

Expression of ovarian tumour suppressor OPCML in the female CD-1 mouse reproductive tract.
MedLine Citation:
PMID:  19176311     Owner:  NLM     Status:  MEDLINE    
Opioid binding protein/cell adhesion molecule-like gene (OPCML) is frequently inactivated in epithelial ovarian cancer, but the role of this membrane protein in normal reproductive function is unclear. The ovarian surface epithelium (OSE) is thought to be the cell of origin of most epithelial ovarian cancers, some of which arise after transformation of OSE cells lining ovarian inclusion cysts, formed during ovulation. We used immunohistochemistry, immunoblotting and quantitative RT-PCR (qRT-PCR) to investigate OPCML expression in the uteri and ovaries of cycling 3-month CD-1 mice, as well as in ovaries from older mice containing inclusion cysts derived from rete ovarii tubules. Immunoblotting showed OPCML bands in uterine, but not whole ovarian or muscle extracts. Strong OPCML immunoreactivity was observed in oviduct, rete ovarii and uterus, whereas in ovary more immunoreactivity was seen in granulosa cells than OSE. No staining was observed in OSE around ovulation sites, where OSE cells divide to cover the site. OPCML immunoreactivity was also weaker in more dysplastic cells lining large ovarian inclusion cysts, compared with normal rete ovarii. No significant changes in Opcml mRNA expression were observed in whole ovarian and uterine extracts at different stages of the cycle. We conclude that murine OPCML is more consistently expressed in cells lining the uterus, oviduct and rete ovarii than in ovary and is not expressed in OSE associated with ovulation sites. This observation supports the hypothesis that a proportion of epithelial ovarian cancers arise from ductal cells and other epithelia of the secondary Mullerian system, rather than the OSE.
Jean S Fleming; H James McQuillan; Melanie J Millier; Grant C Sellar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-27
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  137     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-02     Completed Date:  2012-01-17     Revised Date:  2013-03-27    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  721-6     Citation Subset:  IM    
Department of Anatomy and Structural Biology, University of Otago School of Medical Sciences, Dunedin 9054, New Zealand.
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MeSH Terms
Cell Adhesion Molecules / metabolism*
GPI-Linked Proteins / metabolism
Genitalia, Female / metabolism*
Real-Time Polymerase Chain Reaction
Grant Support
A5009//Cancer Research UK; //Cancer Research UK
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/GPI-Linked Proteins; 0/Opcml protein, mouse

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