Document Detail


Expression on outer membranes of mannose residues, which are involved in osteoclast formation via cellular fusion events.
MedLine Citation:
PMID:  8021265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteoclast, the bone-resorbing cell, is formed from hematopoietic precursors via cell-cell fusion. To evaluate the possibility that under certain specific conditions mannose residues may be expressed on the mammalian cell surface, we examined the action of pradimicin derivatives, which bind specific sugars such as the mannose residue, on the formation of osteoclast induced in the coculture of mouse spleen cells with mouse stromal cells, a process in which cell-cell fusion is involved. Osteoclast formation was inhibited by treatment of this coculture system with pradimicin at the later stage (day 4-7), and this inhibition was specifically abrogated by mannose-rich yeast mannan. During the 8-day cocultivation, osteoclast formation was blocked by the pradimicin on days 6 and 7, when mononuclear preosteoclasts fused into multinucleated osteoclasts. With an interactive laser cytometer ACAS570, fluorescein isothiocyanate-labeled pradimicin was observed to bind osteoclast progenitors at the fusion stage and to have no binding affinity for osteoclast progenitors at the early stage (day 0-3) or for osteoclasts, which were formed after performing fusion between mononuclear preosteoclasts. These results suggest that mannose residues were expressed on outer membranes of monocytes under pathophysiological conditions and that they were involved in the osteoclast formation via cellular membrane fusion events.
Authors:
T Kurachi; I Morita; T Oki; T Ueki; K Sakaguchi; S Enomoto; S Murota
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  269     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-29     Completed Date:  1994-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17572-6     Citation Subset:  IM    
Affiliation:
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibiotics, Antineoplastic / metabolism,  pharmacology
Binding Sites
Cell Differentiation
Cell Fusion
Cell Line
Cell Membrane / drug effects,  metabolism
Kinetics
Mannose / biosynthesis,  metabolism*
Mice
Osteoclasts / cytology,  drug effects,  metabolism*
Spleen / cytology
Stem Cells / cytology,  metabolism
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 31103-86-3/Mannose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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