| Expression of osteoprotegerin and RANK ligand in breast cancer bone metastasis. | |
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MedLine Citation:
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PMID: 12923331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone destruction is primarily mediated by osteoclastic bone resorption, and cancer cells stimulate the formation and activation of osteoclasts next to metastatic foci. Accumulating evidences indicate that receptor activator of NF-kB ligand (RANKL) is the ultimate extracellular mediator that stimulates osteoclast differentiation into mature osteoclasts. In contrast, osteoprotegerin (OPG) inhibits osteoclast development. In order to elucidate a mechanism for cancer-induced osteoclastogenesis, cells from a human breast cancer line, MDA-MB-231, were directly co-cultured with ST2, MC3T3-E1, or with primary mouse calvarial cells. Osteoclast-like cells and tartarate resistant acid phosphatase (TRAP) activities were then quantitated. We examined these cell lines and samples from breast cancer by RT-PCR for the expressions of OPG and RANKL mRNA. Compared to controls, co-culture of MDA-MB-231 cells with stromal or osteoblastic cells induced an increase in number of osteoclasts and TRAP activities. MDA-MB-231 cells alone or breast cancer samples did not express RANKL mRNA. However, co-culture of these cancer cells with stromal or osteoblastic cells induced RANKL mRNA expression and decreased OPG mRNA expression. These experiments demonstrate that direct interactions between breast cancer and stromal or osteoblastic cells induce osteoclastogenesis in vitro through modulating RANKL expression. |
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Authors:
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Hye-Rim Park; Soo-Kee Min; Hyun-Deuk Cho; Duck-Hwan Kim; Hyung Sik Shin; Young Euy Park |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Korean medical science Volume: 18 ISSN: 1011-8934 ISO Abbreviation: J. Korean Med. Sci. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-08-18 Completed Date: 2004-04-30 Revised Date: 2011-03-16 |
Medline Journal Info:
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Nlm Unique ID: 8703518 Medline TA: J Korean Med Sci Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 541-6 Citation Subset: IM |
Affiliation:
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Department of Pathology, College of Medicine, Hallym University, Chuncheon, Korea. hyerim@chol.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Acid Phosphatase / metabolism Animals Bone Neoplasms / metabolism*, secondary* Breast Neoplasms / pathology* Carrier Proteins / biosynthesis* Cell Differentiation Cell Line, Tumor Cells, Cultured Coculture Techniques Culture Media, Conditioned / pharmacology Glycoproteins / biosynthesis* Humans Isoenzymes / metabolism Male Membrane Glycoproteins / biosynthesis* Mice Mice, Inbred C57BL Neoplasm Metastasis Osteoblasts / metabolism Osteoclasts / metabolism Osteoprotegerin Protein Binding RANK Ligand RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Receptors, Cytoplasmic and Nuclear / biosynthesis* Receptors, Tumor Necrosis Factor Reverse Transcriptase Polymerase Chain Reaction Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Culture Media, Conditioned; 0/Glycoproteins; 0/Isoenzymes; 0/Membrane Glycoproteins; 0/Osteoprotegerin; 0/RANK Ligand; 0/RNA, Messenger; 0/Receptor Activator of Nuclear Factor-kappa B; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Tumor Necrosis Factor; 0/TNFRSF11A protein, human; 0/TNFRSF11B protein, human; 0/TNFSF11 protein, human; 0/Tnfrsf11a protein, mouse; 0/Tnfrsf11b protein, mouse; 0/Tnfsf11 protein, mouse; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Korean Med Sci Journal ID (pmc): jkms ISSN: 1011-8934 ISSN: 1598-6357 Publisher: Korean Academy of Medical Sciences |
Article Information Download PDF  Print publication date: Month: 8 Year: 2003 Volume: 18 Issue: 4 First Page: 541 Last Page: 546 ID: 3055084 PubMed Id: 12923331 Publisher Item Identifier: 200308541 |
| Expression of osteoprotegerin and RANK ligand in breast cancer bone metastasis. | |
| Hye-Rim Park | Email: hyerim@chol.com |
| Soo-Kee Min | |
| Hyun-Deuk Cho | |
| Duck-Hwan Kim | |
| Hyung Sik Shin | |
| Young Euy Park | |
| Department of Pathology, College of Medicine, Hallym University, Chuncheon, Korea. |
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