| Expression of the orphan receptor steroidogenic factor-1 mRNA in the rat medial basal hypothalamus. | |
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MedLine Citation:
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PMID: 9030699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Steroidogenic factor-1 (SF-1), an orphan receptor of the nuclear hormone receptor family, binds to the AAGGTCA motif in the promoter elements of several diverse target genes, including some that mediate steroidogenesis and sexual differentiation. In addition, SF-1 is expressed in embryonic forebrain, suggesting that it plays a role in neural development. This study was undertaken to study the distribution and regulation of SF-1 mRNA expression in the rat brain. SF-1 mRNA levels were measured in tissue dissections by ribonuclease protection assay. A 452 nt 32P-labeled cRNA probe, complementary to the putative ligand-binding domain of the rat SF-1 mRNA, was synthesized from the rat SF-1 cDNA inserted into pBluescript II KS, using a Sty 1 fragment and T3 polymerase. The probe protected a single 390 nt transcript in the medial basal hypothalamus (MBH) and peripheral steroidogenic tissues of the male rat. The size of this protected band corresponded to that of the protected sense RNA standard (HindIII fragment of the SF-1 cDNA transcribed with T7 polymerase). No SF-1 mRNA was detected in the preoptic area, amygdala or cingulate cortex. The levels of SF-1 mRNA in MBH were not affected by gonadectomy or androgen treatment, nor was there a sex difference in its expression in adults. In situ hybridization histochemistry revealed that SF-1 was localized to the ventromedial nucleus of the adult hypothalamus. The levels of SF-1 mRNA were high on gestational day 18 after which they fell by approximately 30% and remained constant throughout gestation, the first week of neonatal life, and into adulthood. These results demonstrate that the gene encoding SF-1 is expressed in a discrete region of the rat hypothalamus and appears to be developmentally regulated, but not affected by gonadal hormones in adults. |
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Authors:
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C E Roselli; E Z Jorgensen; M W Doyle; O K Rønnekleiv |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Brain research. Molecular brain research Volume: 44 ISSN: 0169-328X ISO Abbreviation: Brain Res. Mol. Brain Res. Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-05-22 Completed Date: 1997-05-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8908640 Medline TA: Brain Res Mol Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 66-72 Citation Subset: IM |
Affiliation:
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Department of Physiology and Pharmacology, The Oregon Regional Primate Research Center, Oregon Health Sciences University, Portland 97201-3090, USA. rosellic@ohsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Analysis of Variance Animals DNA-Binding Proteins / metabolism* Female Fushi Tarazu Transcription Factors Homeodomain Proteins Hypothalamus, Middle / metabolism* Male RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Receptors, Cell Surface / metabolism* Receptors, Cytoplasmic and Nuclear Sex Factors Steroidogenic Factor 1 Transcription Factors / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Fushi Tarazu Transcription Factors; 0/Homeodomain Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Cytoplasmic and Nuclear; 0/Steroidogenic Factor 1; 0/Transcription Factors; 0/steroidogenic factor 1, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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