Document Detail


Expression ofNeurogenin 1 in mouse embryonic stem cells directs the differentiation of neuronal precursors and identifies uniquepatterns of down-stream gene expression.
MedLine Citation:
PMID:  23288605     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Delineating the cascades of growth and transcription factor expression that shape the developing nervous system will improve our understanding of its molecular histogenesis and suggest strategies for cell replacement therapies. In the current investigation, we examined the ability of the proneural gene, Neurogenin1 (Neurog1; also Ngn1, Neurod3), to drive differentiation of pluripotent embryonic stem cells (ESC). RESULTS: Transient expression of Neurog1 in ESC was sufficient to initiate neuronal differentiation, and produced neuronal subtypes reflecting its expression pattern in vivo. To begin to address the molecular mechanisms involved, we employed microarray analysis to identify potential down-stream targets of Neurog1 expressed at sequential stages of neuronal differentiation. CONCLUSIONS: ESC expressing Neurogenin1 begin to withdraw from cycle and form precursors that differentiate exclusively into neurons. This work identifies unique patterns of gene expression following expression ofNeurog1, including genes and signaling pathways involved in process outgrowth and cell migration, regional differentiation of the nervous system, and cell cycle.
Authors:
J M Velkey; K S O'Shea
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  -     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, 3051 BSRB, Ann Arbor, MI 48109-0616; Department of Cell Biology, Duke University, 454 Davison, 3005 DUMC, Durham, NC, 27710.
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