| Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant. | |
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MedLine Citation:
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PMID: 18079165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fission yeast lsd1/fas2 strain carries a temperature-sensitive mutation of the fatty-acid-synthase alpha-subunit, exhibiting an aberrant mitosis lsd phenotype, with accumulation of very-long-chain fatty-acid-containing phospholipid (VLCFA-PL). A novel 90-kDa protein, Lsd90 (SPBC16E9.16c), was found to be newly expressed in small particle-like structures in lsd1/fas2 cells under restrictive conditions. Two mismatches leading to a double frame shift were found between the sequences of the lsd90(+) gene registered in the genomic database and the sequences determined experimentally at the amino acid, cDNA and genomic DNA levels. Unexpectedly, overexpression and disruption of the lsd90(+) gene in either lsd1/fas2 or wild-type cells did not affect either cell growth or expression of the lsd phenotype. The amounts of VLCFA-PL that accumulated in lsd90-overexpressing lsd1/fas2 cells were significantly lower than those in lsd1/fas2 cells, suggesting the involvement of Lsd90 in the metabolism of VLCFA-PL. |
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Authors:
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Kazuaki Yokoyama; Miyuki Nakagawa; Masayuki Satoh; Shigeaki Saitoh; Naoshi Dohmae; Ayako Harada; Noriko Satoh; Ken Karasawa; Koji Takio; Mitsuhiro Yanagida; Keizo Inoue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-13 |
Journal Detail:
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Title: Journal of biochemistry Volume: 143 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-03 Completed Date: 2008-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 369-75 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, Teikyo University, Suarashi, Sagamiko-machi, Sagamihara-shi, Kanagawa, Japan. yokoyama@pharm.teikyo-u.ac.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB012755 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Fatty Acids / metabolism* Gene Expression Regulation, Fungal Mitosis* Molecular Sequence Data Molecular Weight Mutation / genetics* Phenotype Phospholipids / metabolism* Protein Transport Proteome / analysis RNA, Messenger / genetics, metabolism Schizosaccharomyces / cytology*, genetics, metabolism* Schizosaccharomyces pombe Proteins / chemistry, genetics, metabolism* Sequence Analysis, Protein Subcellular Fractions / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids; 0/Proteome; 0/RNA, Messenger; 0/Schizosaccharomyces pombe Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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