| Expression of nitric oxide synthase and effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles. | |
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MedLine Citation:
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PMID: 14688153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Nitric oxide (NO) is a cell messenger with multiple actions in different biological systems, implicated in the control of follicle and oocyte function. NO is formed from L-arginine by isoforms of nitric oxide synthase (NOS) via NG-hydroxy-L-arginine, with L-citrulline as a byproduct. This study aimed to show how modulation of NO by manipulating NOS substrates would affect mouse follicle growth and ovulation in vitro, where vascular effects of NO are attenuated. METHODS: Immunohistochemistry [endothelial (eNOS) and inducible (iNOS)] and in situ hybridization (iNOS) were applied on mouse ovaries. Cultured follicles were also stained for iNOS by immunohistochemistry. For follicles cultured in the presence or absence of L-arginine, the ability of L-citrulline or NG-hydroxy-L-arginine to substitute for L-arginine was assessed in terms of follicle growth and ovulation. RESULTS: iNOS and eNOS were localized in oocytes and theca, with some staining in granulosa. iNOS mRNA occurred predominantly in granulosa and oocyte. Omission of L-arginine significantly reduced follicle survival and ovulation. Partial compensation for L-arginine withdrawal was achieved with L-citrulline and NG-hydroxy-L- arginine. Specific abnormalities of follicle growth were noted. CONCLUSIONS: NOS is present in mouse follicles, and its action is necessary at a local level for normal follicle development in vitro. Reduced growth, persistent basement membranes and reduced ovulation were associated with in vitro disruption of NO. |
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Authors:
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Leila M Mitchell; C Richard Kennedy; Geraldine M Hartshorne |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 19 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2003-12-22 Completed Date: 2004-08-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 30-40 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / administration & dosage, analogs & derivatives*, pharmacology* Citrulline / administration & dosage, pharmacology* Culture Media / chemistry Dose-Response Relationship, Drug Female Mice Mice, Inbred Strains Nitric Oxide / metabolism* Nitric Oxide Synthase / genetics, metabolism* Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Ovarian Follicle / drug effects, metabolism* Ovulation / drug effects RNA, Messenger / metabolism Tissue Survival Transferrin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/RNA, Messenger; 10102-43-9/Nitric Oxide; 11096-37-0/Transferrin; 372-75-8/Citrulline; 53054-07-2/N(omega)-hydroxyarginine; 74-79-3/Arginine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos2 protein, mouse; EC 1.14.13.39/Nos3 protein, mouse |
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