| Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion. | |
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MedLine Citation:
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PMID: 18755801 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The composition of the beta-cell exocytic machinery is very similar to that of neuronal synapses, and the developmental pathway of beta-cells and neurons substantially overlap. beta-Cells secrete gamma-aminobutyric acid and express proteins that, in the brain, are specific markers of inhibitory synapses. Recently, neuronal coculture experiments have identified three families of synaptic cell-surface molecules (neurexins, neuroligins, and SynCAM) that drive synapse formation in vitro and that control the differentiation of nascent synapses into either excitatory or inhibitory fully mature nerve terminals. The inhibitory synapse-like character of the beta-cells led us to hypothesize that members of these families of synapse-inducing adhesion molecules would be expressed in beta-cells and that the pattern of expression would resemble that associated with neuronal inhibitory synaptogenesis. Here, we describe beta-cell expression of the neuroligins, neurexins, and SynCAM, and show that neuroligin expression affects insulin secretion in INS-1 beta-cells and rat islet cells. Our findings demonstrate that neuroligins and neurexins are expressed outside the central nervous system and help confer an inhibitory synaptic-like phenotype onto the beta-cell surface. Analogous to their role in synaptic neurotransmission, neurexin-neuroligin interactions may play a role in the formation of the submembrane insulin secretory apparatus. |
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Authors:
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Arthur T Suckow; Davide Comoletti; Megan A Waldrop; Merrie Mosedale; Sonya Egodage; Palmer Taylor; Steven D Chessler |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-08-28 |
Journal Detail:
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Title: Endocrinology Volume: 149 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-03-31 Revised Date: 2013-03-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 6006-17 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, California 92093, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Animals Blotting, Western Brain / metabolism Carrier Proteins / genetics, metabolism Cell Adhesion Molecules, Neuronal / genetics, metabolism* Cell Line, Tumor Glycoproteins / genetics, metabolism Guanylate Kinase / genetics, metabolism Humans Insulin / secretion* Insulin-Secreting Cells / metabolism* Membrane Proteins / genetics, metabolism Microscopy, Confocal Nerve Tissue Proteins / genetics, metabolism* Neuropeptides / genetics, metabolism RNA Interference Rats Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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5P30 AI36214/AI/NIAID NIH HHS; DK063491-4S1/DK/NIDDK NIH HHS; P30 NS047101/NS/NINDS NIH HHS; R01 DK080971-04/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cell Adhesion Molecules, Neuronal; 0/Glycoproteins; 0/Insulin; 0/Membrane Proteins; 0/NLGN4X protein, human; 0/Nerve Tissue Proteins; 0/Neuropeptides; 0/gephyrin; 0/neurexin II; 0/neurexophilin; 0/neuroligin 1; 0/neuroligin 2; 0/neuroligin 3; 156532-79-5/neurexin IIIbeta; 156532-80-8/neurexin Ibeta; EC 2.7.4.-/CASK kinases; EC 2.7.4.8/Guanylate Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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