| Expression of the neural specific protein, GAP-43, dramatically lengthens the cell cycle in fibroblasts. | |
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MedLine Citation:
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PMID: 19589379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been demonstrated that during neurogenesis in the mammalian brain, cell-cycle lengthening in neuronal progenitors may cause them to switch from proliferation to neuron-generating division. However, little is known about the cellular mechanisms involved in lengthening of the cell cycle. Growth-associated protein-43 (GAP-43) is a nervous system-specific protein whose expression in proliferating neuroblasts is related to neurogenesis. In this study, we investigated the effect of GAP-43 on cell-cycle progression in transgenic fibroblast cells. Using cumulative bromodeoxyuridine labeling, cell-cycle kinetics in GAP-43-transgenic and control NIH 3T3 cells were analyzed. Our data demonstrate that expression of GAP-43 in fibroblasts results in lengthening of the cell cycle compared to control fibroblasts. The mechanism by which GAP-43 mediated this effect appeared to involve increasing the time spent by the cells in the G(1) phase of the cell cycle. |
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Authors:
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Junpeng Zhao; Yajuan Yao; Changlei Xu; Xiaohua Jiang; Qunyuan Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-07 |
Journal Detail:
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Title: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience Volume: 27 ISSN: 1873-474X ISO Abbreviation: Int. J. Dev. Neurosci. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-18 Completed Date: 2009-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8401784 Medline TA: Int J Dev Neurosci Country: England |
Other Details:
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Languages: eng Pagination: 531-7 Citation Subset: IM |
Affiliation:
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Beijing Institute for Neuroscience, Beijing Center for Neural Regeneration and Repair, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing 100069, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine Cell Cycle / genetics* Cell Proliferation Fibroblasts / cytology, metabolism* G1 Phase / genetics GAP-43 Protein / genetics, metabolism* Gene Expression Regulation, Developmental / genetics Mice NIH 3T3 Cells Neurogenesis / genetics* Neurons / metabolism* Stem Cells / metabolism* Transgenes / genetics |
| Chemical | |
Reg. No./Substance:
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0/GAP-43 Protein; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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